Oil cs2 hc plan apo

Huh7 and HepG2 cells were seeded on 13 mm coverslips in 12-well plates. After washing with PBS, cells were fixed for 10 min with 3.7% formaldehyde in PBS, subsequently washed with PBS, permeabilized and blocked with 10% FBS, 0.1% Triton X-100 in PBS at 37 °C. After incubation with the primary antibody (anti-CAD, Abcam, Cambridge, UK, ab40765, rabbit monoclonal, 1:100) in blocking solution at RT for 1 h and a brief wash with 0.1% Triton X-100 in PBS, cells were incubated with Alexa Fluor® 488 goat anti-rabbit IgG, MitoView 633 (1:1000), and the nuclear dye DAPI in 1% FBS, 0.1% Triton X-100 in PBS at RT for 1 h. After an additional washing step with 0.1% Triton X-100 in PBS, cells were mounted with MOWIOL, and images were obtained with a Leica SP8 confocal microscope using either a 40× 1.30 NA Oil CS2 HC Plan Apo or a 63× 1.40 NA Oil CS2 HC Plan Apo objective operating at 25 °C.