Facs caliber cytometer

Manufactured by BD

Sourced in United States

The BD FACS Caliber is a flow cytometry instrument designed for cell analysis. It utilizes laser technology to detect and measure the characteristics of individual cells within a sample. The core function of the FACS Caliber is to provide researchers with the ability to analyze cell populations based on their size, granularity, and expression of specific fluorescently-labeled markers.

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Close spelling variants of this product

FACS Caliber flow cytometer (317 citations) FACS caliber 101 flow cytometer (2 citations) FACS) caliber bench-top flow cytometer (3 citations) FACS Caliber ow cytometer (3 citations) FACS Caliber (612 citations) BD Caliber (15 citations) BD FACSTM (2 citations)

32 protocols using facs caliber cytometer

Multiparameter Flow Cytometry Analysis

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For PVR/CD155 and PD-L1 staining, the cultured 4T1, CT26, MC38, and H22 cells were harvested and stained with monoclonal antibodies against mouse PVR/CD155 (PE; Clone TX56, Cat# 131507, Biolegend, USA) or PD-L1 (PE; Clone 10F.9G2, Cat# 124308, Biolegend, USA) respectively for 15 min at room temperature and immediately analyzed with a FACS Caliber cytometer (BD Biosciences, USA).
Ascites fluid was collected from the peritoneal cavity of the mice. After counting the cells, 2×10⁷ single-cell suspensions were stained with monoclonal antibodies against mouse CD45 (APC; Clone 30-F11, Cat# 559864, BD), CD3 (FITC; Clone 17A2, Cat# 100204 or PE; Clone 17A2, Cat# 100206, Biolegend, USA), CD8 (PerCP-Cy5.5; Clone 53-6.7, Cat# 551162, BD), CD49b (PerCP-Cy5.5; Clone HMα2, Cat# 103520, Biolegend), NK1.1 (FITC; Clone PK136, Cat# 11-5941-82, Ebioscience, USA) and TIGIT (PE; Clone 1G9, Cat# 142104, Biolegend) for 15 min at room temperature. Cells were fixed with 4% paraformaldehyde (PFA; Cat# 1004965000, Sigma-Aldrich, Germany) and analyzed with a FACS Caliber cytometer (BD). Data analyses were performed with FlowJo software (Treestar, USA).

Zuo S., Wei M., He B., Chen A., Wang S., Kong L., Zhang Y., Meng G., Xu T., Wu J., Yang F., Zhang H., Wang S., Guo C., Wu J., Dong J, & Wei J. (2021). Enhanced antitumor efficacy of a novel oncolytic vaccinia virus encoding a fully monoclonal antibody against T-cell immunoglobulin and ITIM domain (TIGIT). EBioMedicine, 64, 103240.

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Cell Cycle Analysis by FACS

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Cell cycles were analyzed after staining with propidium iodide (PI) on a FACS Caliber cytometer (Becton Dickinson, San Jose, CA, USA) as previously described¹⁶ .

Bing S., Xiang S., Xia Z., Wang Y., Guan Z., Che J., Xu A., Dong X., Cao J., Yang B., Wang J., He Q, & Ying M. (2023). AKT inhibitor Hu7691 induces differentiation of neuroblastoma cells. Acta Pharmaceutica Sinica. B, 13(4), 1522-1536.

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Quantifying Cellular Senescence Markers

Cited 1 time

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A stable C2C12 line expressing a p27 sensor (p27-mVenus, a fusion protein consisting of mVenus and a defective mutant of p27-CDKI, (p27K(−)) [57 (link)] was generated, transfected with either control or IFT88 siRNAs, and placed in suspension arrest. After recovery from methyl cellulose medium, these cells were assayed for proportion of mVenus positive cells by flow cytometry using a FACS Caliber cytometer (Becton Dickenson). CelQuest® software was used for acquisition and FlowJo® software was used to analyze the data.

Venugopal N., Ghosh A., Gala H., Aloysius A., Vyas N, & Dhawan J. (2020). The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts. BMC Molecular and Cell Biology, 21, 25.

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Cdh2 Impacts Oct4 Expression in ESCs

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Wild-type- and Cdh2- ESCs were dissociated into single cells by Trypsin-EDTA after 48 hours of treatment, washed twice and diluted in the FACS buffer (2% FBS and 10 mM HEPES in DMEM). The Oct4 deltaPE-GFP positive cell fractions were analyzed by flow cytometry with a FACS Caliber cytometer (Becton Dickinson, Franklin Lakes, NJ). All values are means ± SD of 3 experiments. Statistical significance was evaluated by Student’s t-test using JMP software.

Takehara T., Teramura T., Onodera Y., Frampton J, & Fukuda K. (2015). Cdh2 stabilizes FGFR1 and contributes to primed-state pluripotency in mouse epiblast stem cells. Scientific Reports, 5, 14722.

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Cell Cycle Analysis by Flow Cytometry

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Adherent cells were trypsinized, washed in PBS and pelleted by centrifugation. Suspension-arrested cells were recovered from methylcellulose by dilution with PBS followed by centrifugation as described earlier. Cell pellets were dispersed in 0.75 ml of PBS, and fixed by drop wise addition into 80% ice-cold ethanol with gentle stirring, following which they were briefly washed with PBS and resuspended in PBS with 40 μM of the DNA dye DRAQ5™ (Cat. No DR50050, Biostatus) per 10⁶cells. Cell cycle analysis was performed on a FACS Caliber® Cytometer (Becton Dickenson) using CelQuest® software and analyzed using FlowJo® software. At least 10,000 cells were acquired for each sample. Forward scatter and side scatter were used to gate cell populations and doublets were removed from analysis.

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Phenotypic Characterization of Isolated ADSCs

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To characterize the phenotypes of the isolated ADSCs,
the cultured ADSCs were harvested and stained with
antibodies against CD44, CD73, CD90, CD45, CD11b
and CD31. Cell phenotyping was performed using flow
cytometry analysis by a FACS caliber cytometer (Becton
Dickinson, San Diego, CA, USA).

Aminzadeh A., Tekiyeh Maroof N., Mehrabani M., Bahrampour Juybari K, & Sharifi A.M. (2020). Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats. Cell Journal (Yakhteh), 22(Suppl 1), 38-48.

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Cytotoxicity of siRNA Transfection in ARPE-19 Cells

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Cytotoxicity of siRNA transfection to ARPE-19 cells was determined by examining cell apoptosis using a FACS Caliber cytometer (Becton Dickinson, San Jose, CA, US) according to the manufacturer's instructions. In brief, ARPE-19 cells were plated at 1 × 10⁶ per well in 6-well plates. Cells with ASPP2-siRNA transfection and two control groups were used: normal control cells without transfection treatment (NC) and cells with scrambled control-siRNA transfection (SC). After 48 h incubation, cells were detached using ethylenediaminetetraacetic acid (EDTA), washed in ice-cold PBS, and treated with the FITC Annexin V Apoptosis Detection Kit.

Chen X.L., Bai Y.J., Hu Q.R., Li S.S., Huang L.Z, & Li X.X. (2016). Small Interfering RNA Targeted to ASPP2 Promotes Progression of Experimental Proliferative Vitreoretinopathy. Mediators of Inflammation, 2016, 7920631.

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Cell Cycle Analysis by Flow Cytometry

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Adherent cells were trypsinized, washed in PBS and pelleted by centrifugation. Suspension-arrested cells were recovered from methylcellulose by dilution with PBS followed by centrifugation as described earlier.
Cell pellets were dispersed in 0.75 ml of PBS, and xed by drop wise addition into 80% ice-cold ethanol with gentle stirring, following which they were brie y washed with PBS and resuspended in PBS with 40 μM of the DNA dye DRAQ5 TM (Cat. No DR50050, Biostatus) per 10 6 cells. Cell cycle analysis was performed on a FACS Caliber® Cytometer (Becton Dickenson) using CelQuest® software and analyzed using FlowJo® software. At least 10,000 cells were acquired for each sample. Forward scatter and side scatter were used to gate cell populations and doublets were removed from analysis.

Venugopal N., Ghosh A., Gala H., Aloysius A., Vyas N., & Dhawan J. (2020). The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts.

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Quantifying p27 Expression in C2C12 Cells

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A stable C2C12 line expressing a p27 sensor (p27-mVenus, a fusion protein consisting of mVenus and a defective mutant of p27-CDKI, (p27K(-)) (56) was generated, transfected with either control or IFT88 siRNAs, and placed in suspension arrest. After recovery from methyl cellulose medium, these cells were assayed for proportion of mVenus positive cells by ow cytometry using a FACS Caliber cytometer (Becton Dickenson). CelQuest® software was used for acquisition and FlowJo® software was used to analyze the data.

Venugopal N., Ghosh A., Gala H., Aloysius A., Vyas N., & Dhawan J. (2020). The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts.

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Quantifying p27 Expression in C2C12 Cells

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Venugopal N., Ghosh A., Gala H., Aloysius A., Vyas N., & Dhawan J. (2020). The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts.

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