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Pd l1 apc antibody

Manufactured by BioLegend

The PD-L1-APC antibody is a flow cytometry reagent that binds to the programmed cell death ligand 1 (PD-L1) protein. PD-L1 is a key immune checkpoint molecule that plays a role in regulating T-cell activation and immune responses. The APC (Allophycocyanin) fluorophore conjugated to the antibody enables detection and analysis of PD-L1 expression on cells using flow cytometry.

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2 protocols using pd l1 apc antibody

1

Macrophage Phenotyping by Flow Cytometry

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For the phenotype analysis of Raw264.7 macrophages, cells were first treated as indicated. Then, Raw264.7 cells were harvested and incubated with FITC-conjugated F4/80 antibody (SC-71085, Santa Cruz, CA, USA), PE-conjugated CD206 antibody (141,705, Biolegend, CA, USA), or PE-Cy7-conjugated CD206 antibody (E-AB-F1135H, Elabscience, Houston, TX, USA) for 30 min at 37 °C. For the phenotype analysis of THP1 macrophages, cells were treated as indicated, harvested and incubated with APC-conjugated F4/80 antibody (17-4801-82, Invitrogen) and PE-conjugated CD163 antibody (12-1639-42, eBioscience) for 30 min at 37 °C. For the phenotypic analyses of primary macrophages isolated from mouse 4 T1-Luc xenografts, cells were incubated with CD45-PE-Cy7 (25-0451-82, eBioscience), F4/80-APC antibody (17-4801-82, Invitrogen), and CD206-PE antibody (141,705, Biolegend) for 30 min at 37 °C. For PD-L1 expression analyses of primary macrophages, cells were incubated with CD45-PE-Cy7 (25-0451-82, eBioscience), F4/80-FITC antibody (SC-71085, Santa Cruz), and PD-L1-APC antibody (124,312, Biolegend) for 30 min at 37 °C. After incubation, the cells were washed once with PBS and subjected to flow cytometry analysis.
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2

Immunophenotyping of PAAD Cells

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The PD‐L1‐APC antibody (BioLegend) and Galectin‐9 PE antibody (BD Biosciences) were used to examine the expression of surface biomarkers Galectin‐9 and PD‐L1 on the PAAD cells. The mean fluorescence intensity of each labeling was analyzed. After 48 h, the target cells were identified on a FACS AriaII flow cytometer (BD Biosciences). Detailed descriptions of the methods are provided in Appendix S1.
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