Pd l1 apc antibody

For the phenotype analysis of Raw264.7 macrophages, cells were first treated as indicated. Then, Raw264.7 cells were harvested and incubated with FITC-conjugated F4/80 antibody (SC-71085, Santa Cruz, CA, USA), PE-conjugated CD206 antibody (141,705, Biolegend, CA, USA), or PE-Cy7-conjugated CD206 antibody (E-AB-F1135H, Elabscience, Houston, TX, USA) for 30 min at 37 °C. For the phenotype analysis of THP1 macrophages, cells were treated as indicated, harvested and incubated with APC-conjugated F4/80 antibody (17-4801-82, Invitrogen) and PE-conjugated CD163 antibody (12-1639-42, eBioscience) for 30 min at 37 °C. For the phenotypic analyses of primary macrophages isolated from mouse 4 T1-Luc xenografts, cells were incubated with CD45-PE-Cy7 (25-0451-82, eBioscience), F4/80-APC antibody (17-4801-82, Invitrogen), and CD206-PE antibody (141,705, Biolegend) for 30 min at 37 °C. For PD-L1 expression analyses of primary macrophages, cells were incubated with CD45-PE-Cy7 (25-0451-82, eBioscience), F4/80-FITC antibody (SC-71085, Santa Cruz), and PD-L1-APC antibody (124,312, Biolegend) for 30 min at 37 °C. After incubation, the cells were washed once with PBS and subjected to flow cytometry analysis.