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Optima 2100

Manufactured by PerkinElmer
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The Optima 2100 is an Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) designed for multi-elemental analysis. It is capable of detecting and quantifying a wide range of elements in various sample types.

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Lab products found in correlation

Novocyte flow cytometer, by Agilent Technologies (1 mentions) Tecnai f20, by Thermo Fisher Scientific (1 mentions) Nicolet 6700 ft ir spectrometer, by Thermo Fisher Scientific (1 mentions) Nano z, by Malvern Panalytical (1 mentions) Zetasizer pro, by Malvern Panalytical (1 mentions) Uv 2600i, by Shimadzu (1 mentions) X series 2, by Thermo Fisher Scientific (1 mentions) Suprapur, by Merck Group (1 mentions) Ultrapure concentrated nitric acid, by Merck Group (1 mentions) Elan drc e, by PerkinElmer (1 mentions) Multiwave go, by Anton Paar (1 mentions) Cary 60 uv visible spectrophotometer, by Agilent Technologies (1 mentions) Nanolab g3 cx, by Thermo Fisher Scientific (1 mentions) 100 series, by PerkinElmer (1 mentions) Raman spectrometer, by WITec (1 mentions)

18 protocols using optima 2100

1

Comprehensive Elemental Analysis of Plant Tissues

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The dry material of the root and shoot was digested using the Wolf protocol [67 (link)], and the extract was used to determine the Na, K, Ca, P, Mg and N contents. The phosphorus (P) content was estimated spectrophotometrically [68 ], potassium (K) and sodium (Na) were assessed by the Flame photometer (Jeway, P70, Fuzhou, China) and Ca and Mg by ICP-OES (Optima 2100 DV Perkin-Elmer, Waltham, MA, USA). The nitrogen (N) content was measured according to Bremner [69 (link)].
Shafiq H., Shani M.Y., Ashraf M.Y., De Mastro F., Cocozza C., Abbas S., Ali N., Z.a.i.b.-.u.n.-.N.i.s.a., Tahir A., Iqbal M., Khan Z., Gul N, & Brunetti G. (2024). Copper Oxide Nanoparticles Induced Growth and Physio-Biochemical Changes in Maize (Zea mays L.) in Saline Soil. Plants, 13(8), 1080.
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2

Comprehensive Characterization of Nanomaterials

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The images of GNSs/GNBs were obtained by TEM (JEM-2100, Japan). Specifically, we centrifuged the GNSs/GNBs at 6000 rpm twice and redispersed them in deionized water, then we carefully added the GNSs/GNBs solutions with appropriate concentrations dropwise onto a copper mesh for TEM characterizations. The size distribution was measured from the TEM images by ImageJ software. The dynamic light scattering (DLS) and average ζ potential were measured by a Zetasizer analyzer (Zetasizer-Nano ZS, UK). The ultraviolet-visible (UV-Vis) spectrum of GNSs/GNBs was obtained by a spectrophotometer (China). Flow cytometry was performed by a NovoCyte flow cytometer (Agilent, USA). Inductively coupled plasma mass spectrometry (ICP-MS) was carried out by an Optima 2100 instrument from PerkinElmer (Waltham, USA). Confocal laser scanning microscopy (CLSM) images were captured using a Nikon A1 camera (Tokyo, Japan). The RT-qPCR data was detected using the CFX384 Multiplate RT Fluorescence Quantitative PCR instrument (Bio-Rad, USA).
Zhang R., Li D., Zhao R., Luo D., Hu Y., Wang S., Zhuo X., Iqbal M.Z., Zhang H., Han Q, & Kong X. (2024). Spike structure of gold nanobranches induces hepatotoxicity in mouse hepatocyte organoid models. Journal of Nanobiotechnology, 22, 92.
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3

Inductively Coupled Plasma Mass Spectrometry for Pb Analysis

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The dried plants were retrieved, crushed, and ground, and the powder was placed in a beaker. About 7 mL of nitric acid and 2 mL of hydrogen peroxide were added to the beaker, which was then placed in a microwave oven at 170°C for 30 min. The homogenate was then retrieved, and transferred to a beaker with polytetrachloroacetic acid containing 20% dilute nitric acid by volume. To remove the acid content, the mixture was heated at 170°C on an electric heating pad until it was almost dry. The residue was then transferred to a 25 mL bottle and the volume was adjusted using 20% dilute nitric acid by weight. Finally, an Optima 2100 DV inductively coupled plasma mass spectrometer (PerkinElmer, USA) was used to conduct a full-scale analysis on Pb ions.
Zhou J., Zhang Z., Zhang Y., Wei Y, & Jiang Z. (2018). Effects of lead stress on the growth, physiology, and cellular structure of privet seedlings. PLoS ONE, 13(3), e0191139.
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4

Physicochemical Characterization of TiO2 Nanoprobes

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The concentration of Ti element was measured by inductively coupled plasma optical emission spectrometry (ICP-OES) (Optima 2100, PerkinElmer). Mean size and zeta potential of bTiO2-PEG-p, bTiO2-HA-p and wTiO2-HA-p nanoprobes were performed by Particle Size-Zeta Potential Analyzer (Nano ZS, Malvern Instruments Ltd, England). The morphology of nanoprobes was analyzed by transmission electron microscopy (TEM, Tecnai F20, Thermo Fisher Scientific, USA). Ultraviolet visible (UV–vis) absorption spectra were determined by a UV–visible spectrophotometer (T10CS, Persee General Equipment Co., Ltd, China). Fourier transform infrared (FTIR) spectrum was obtained from a Nicolet 6700 FTIR spectrometer (Thermo Scientific, USA) in the range of 400–4000 cm−1.
Dai T., He W., Tu S., Han J., Yuan B., Yao C., Ren W, & Wu A. (2022). Black TiO2 nanoprobe-mediated mild phototherapy reduces intracellular lipid levels in atherosclerotic foam cells via cholesterol regulation pathways instead of apoptosis. Bioactive Materials, 17, 18-28.
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5

Salivary Elemental Profiling in Leukemia

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Trace elements were determined using an inductively coupled plasma optical emission spectroscopy-Optima 2100 (ICP-OES, PerkinElmer, Shelton, CT, USA). This is a highly sensitive technique that allows the determination of many elements in volumes of minimal biological samples [22 (link)]. Thus, the purpose was to assess the relationship between the levels of the elements of the salivary state in patients with different leukemia types compared to batches of healthy and artificial controls. In ICP-OES, samples were introduced as dilute solutions of frozen saliva and freeze-dried after the 7-day immersion of the dental composites for different elements (Zn, Ca, P, Fe, Cu, K, Mg, Na) present in the saliva composition.
Mester A., Moldovan M., Cuc S., Petean I., Tomuleasa C., Piciu A., Dinu C., Bran S, & Onisor F. (2022). Structural Changes in Resin-Based Composites in Saliva of Patients with Leukemia before Starting Chemotherapeutic Regimen. Polymers, 14(3), 569.
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6

Quantification of Zinc in Cells

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The protocols for detection of Zn content in cell were reported in our previous paper [41 (link)]. Briefly, isolated GCs were cultured in 6-well plates for 2 days then the cells were treated with different concentration of ZnO NP or ZnSO4 for 24h. About 2×106 cells per treatment were collected for determination of Zn in the cells. 500μL of 0.4% Triton X-100 in PBS was used to lyse the cells and the lysate was diluted to 2mL with 0.1% Triton X-100. Samples were analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES, Optima 2100, Perkin-Elmer, Shelton, CT, USA) [46 ].
Zhao Y., Li L., Min L.J., Zhu L.Q., Sun Q.Y., Zhang H.F., Liu X.Q., Zhang W.D., Ge W., Wang J.J., Liu J.C, & Hao Z.H. (2016). Regulation of MicroRNAs, and the Correlations of MicroRNAs and Their Targeted Genes by Zinc Oxide Nanoparticles in Ovarian Granulosa Cells. PLoS ONE, 11(5), e0155865.
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7

Nanoparticle Characterization Protocol

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The morphology of nanoparticles was verified by TEM (Talos F200X). The dynamic light scattering (DLS) and zeta potential was carried out by Zetasizer Pro (Malvern Panalytical). The UV–vis spectra were characterized by UV–vis spectrophotometer (UV-2600i, Shimadzu Corporation). The Mn content was detected by ICP-OES (Optima 2100, PerkinElmer). XPS analysis was conducted by an Axis Ultra DLD instrument. The dissolved oxygen was performed on an oxygen probe (ST300 D, OHAUS).
Pan Y., Yu L., Liu L., Zhang J., Liang S., Parshad B., Lai J., Ma L.M., Wang Z, & Rao L. (2024). Genetically engineered nanomodulators elicit potent immunity against cancer stem cells by checkpoint blockade and hypoxia relief. Bioactive Materials, 38, 31-44.
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8

Quantifying Cellular Trace Elements

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After 24 h treatment with ZnO NPs, ZnSO4, CuO NPs or SiO2 NPs, cells (2 × 106 cells/treatment) were lysed in 500 μL of 0.4% Triton X-100 in PBS, then the lysate was diluted to 2mL with 0.1% Triton X-100. Samples were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES, Optima 2100, Perkin-Elmer, Shelton, CT, USA) [81 (link)]. The voltage for the ion lens was set at 6 V; the gas flow rate in the spray chamber was 0.88 L/min; the power output for the RF generator was 1100 W; the auxiliary gas flow rate was 1.2 L/min; and the nebulizer gas flow rate of the plasma was 16 L/min. All the certified reference materials (in solution) were purchased from the National Institute of Metrology (Beijing, China). Blank controls underwent the same procedures. All procedures were performed in triplicate [81 (link)].
Liu J., Zhao Y., Ge W., Zhang P., Liu X., Zhang W., Hao Y., Yu S., Li L., Chu M., Min L., Zhang H, & Shen W. (2017). Oocyte exposure to ZnO nanoparticles inhibits early embryonic development through the γ-H2AX and NF-κB signaling pathways. Oncotarget, 8(26), 42673-42692.
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9

ICP-OES Analysis of Cellular Zinc

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The GCs were plated in 6-well plates and grown for 2 days then the cells were treated with different concentration (based on Zn; 1, 5 and 10μg/ml) of ZnO NP or ZnSO4 for 24h. About 2×106 cells/treatment were lysed in 500μL of 0.4% Triton X-100 in PBS, then the lysate was diluted to 2mL with 0.1% Triton X-100. Samples were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES, Optima 2100, Perkin-Elmer, Shelton, CT, USA) [25 (link)].
Zhao Y., Li L., Zhang P.F., Shen W., Liu J., Yang F.F., Liu H.B, & Hao Z.H. (2015). Differential Regulation of Gene and Protein Expression by Zinc Oxide Nanoparticles in Hen’s Ovarian Granulosa Cells: Specific Roles of Nanoparticles. PLoS ONE, 10(10), e0140499.
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10

Adsorption of U(VI) on PO4/PE

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To
evaluate the effect of −PO4 group functionalization
by the Ar-jet plasma treatment on the adsorption capability of PE
toward U(VI), U(VI) adsorption on PO4/PE and PE was studied
by the batch adsorption technique. After the adsorbent and salinity
(such as NaCl) were pre-equilibrated for 24 h, the U(VI) solution
(UO2CO3) and Milli-Q water were added to achieve
the desired components and the pHs of the suspensions were adjusted
by the corresponding acid and basic solutions. After shaking for 48
h, the supernatant was filtered by 0.45 μm membrane filters.
The final U(VI) concentrations (of mg/L and μg/L levels) in the supernatants
were measured on an Optima 2100 DV inductively coupled plasma (ICP)
atomic emission spectroscopy system (Perkin Elmer) and on an ICP mass
spectroscopy (ICP-MS, Thermo Scientific X-Series II) system, respectively.
Shao D., Li Y., Wang X., Hu S., Wen J., Xiong J., Asiri A.M, & Marwani H.M. (2017). Phosphate-Functionalized Polyethylene with High Adsorption of Uranium(VI). ACS Omega, 2(7), 3267-3275.
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