Microwell array plates were used to generate large numbers (>105) of uniform EBs via forced aggregation. The microwells, in the form of inverted pyramids with 400-μm base width, were arranged in a six-well plate format and manufactured via polydimethylsiloxane (PDMS) molding. The following process was used to facilitate demolding and ensure casting compatibility between polymers. Briefly, SYLGARD 184 (Dow Corning) was mixed 10:1 with its cross-linker and poured into a commercially available microwell plate, degassed in a vacuum chamber, and cured at 80°C for 2 hours to generate a negative cast of the microwells. Next, Ecoflex 00-30 (Smooth-On Inc.) was used to create a positive mold by following a similar method of pouring, degassing, and curing as for the SYLGARD 184. After removing the cured Ecoflex, a two-part polyurethane (Smooth-On Inc.) was poured onto the Ecoflex positive to create a polyurethane-negative mold. Next, ~80 g of SYLGARD 184 was mixed with 8 g of its cross-linker in a planetary mixer, poured into a polyurethane negative mold, degassed for 10 min in a vacuum, and allowed to cure at room temperature for 24 to 48 hours. The cured SYLGARD 184 was then placed in the oven at 80°C for >2 hours to ensure complete cross-linking before removal from the polyurethane mold.
Ecoflex
Manufactured by Smooth-On
Sourced in United States
Ecoflex is a two-part silicone rubber compound designed for making flexible, durable molds and parts. It is a user-friendly and versatile material that cures at room temperature. Ecoflex is available in different shore hardness levels to suit various applications.
Automatically generated - may contain errors
Lab products found in correlation
Sylgard 184, by Dow (3 mentions)
Sylgard 170, by Dow (2 mentions)
Dragon skin 10, by Smooth-On (1 mentions)
Objet350 connex3 printer, by Stratasys (1 mentions)
Ar g1l, by Keyence (1 mentions)
Tangoplus, by Stratasys (1 mentions)
Tangoblackplus, by Stratasys (1 mentions)
Methanol, by Maclin Biochemical Technology (1 mentions)
Citric acid, by Maclin Biochemical Technology (1 mentions)
Aniline, by Maclin Biochemical Technology (1 mentions)
Ecoflex 00 30, by Smooth-On (1 mentions)
Sylgard 184, by Smooth-On (1 mentions)
Sylgard 184 kit, by Dow (1 mentions)
Mwcnts, by XFNANO (1 mentions)
12 protocols using ecoflex
1
Microwell Array for Uniform Embryoid Body Generation
2
Fabrication of Ecoflex-based Triboelectric Sensor
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Firstly, the Ecoflex (volume ratio of A: B, 20mL, Smooth-on, Inc., Macungie, PA, USA) was spin-coated on a glass substrate at a speed of 300 rpm for 20 s and cured at 120 °C for 3 h. A copper (Cu) conductive tape was pasted on the back of the Ecoflex with a thickness of 1 mm. The Cu electrode and Ecoflex film acted as the bottom electrode and triboelectric layer. The MXene/GO film was adhered to a acrylic plate (3M Company, Saint Paul, MN, USA) with a diameter of 6 cm. The two films were connected to the two separated plates of a test instrument.
3
Fabrication of Soft Actuator Prototypes
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Two methods and four materials were used to fabricate the soft actuators. Two methods are: (1) the traditional casting process, (2) 3D printing using the Objet350 Connex3 printer (Stratasys, Minnesota, USA) and the Agilista printer (Keyence, Japan). Four materials are: (1) the Dragon Skin 10 (Smooth-on Inc., PA, USA), (2) the Ecoflex (Smooth-on Inc., PA, USA), (3) the TangoPlus or TangoBlackPlus (Stratasys, MN, USA), which mainly consists of propenoic acid, ethyl ester, and trimethylbicyclo, and has a hardness of Shore A26-A28 and an elongation at break of 170–220%, and (4) the AR-G1L (Keyence, Japan), which mainly consists of silicone and acrylate monomer, and has a hardness of Shore A35 and an elongation at break of 160%.
4
Sustainable Textile-Based Functional Sensors
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PP SF was obtained from Hangzhou Elsite Industrial Co., Ltd (its areal density is about 71 g m−2). Aniline (99.5%), phytic acid (50%), polyvinyl butyraldehyde (PVB), citric acid, methanol, disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O), potassium chloride (KCl), calcium chloride (CaCl2), magnesium chloride (MgCl2), and ammonium chloride (NH4Cl) were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). Sulfuric acid (H2SO4), sodium chloride (NaCl), hydrochloric acid and sodium hydroxide (NaOH) were purchased from Aladdin Industrial Corporation (Shanghai, China). Silver/silver chloride (Ag/AgCl) paste was bought from Shanghai Julong Electronic-technology Co., Ltd. Ecoflex was purchased from Smooth-On (U.S.A). Carbon conductive ink was purchased from Jiewei Screen Printing Co., Ltd (Shenzhen, China). OPU was purchased from Haobo Waterproof Material Co., Ltd. All materials were used as received without any further purification. The screen-printing mask was customized by Dabaicai Screen Printing Co., Ltd. Deionized water (DI) was employed in all experiments.
5
Fabrication of Liquid Metal Elastomer Composites
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Nickel microparticles (2–5 µm) from Beijing Deke Island Gold Technology Co., Ltd., and silicone of Ecoflex 00–30 from Smooth‐On were used. The eutectic Bi31.6In48.8Sn19.6 alloy (EBiInSn) is composed of 31.6 wt% bismuth, 48.8 wt% indium, and 19.6 wt% tin. The metal elements of Ga, Bi, In, and Sn were supplied by Zhuzhou Yilong Hung Industrial Co. Ltd, which was mixed by the given proportion at 200 °C in a vacuum drying oven for 4 h to prepare BiInSn. The elastomer composite (EC) was prepared through mixing BiInSn and Ni particles into parts of Ecoflex00–30 A and Ecoflex00–30 B separately using an electric mortar with 2500 rpm for 20–30 min at 70 °C. The liquid BiInSn was easily stirred to fabricate microliquid metal droplets with sizes 10–20 µm. The prepared silicone composites of parts A and B were then mixed by an electric mortar with 100 rpm for 3–5 min, which were then filled into LMF and cured in a vacuum drying oven for 5 h at 50 °C to obtain LMF‐EC. All the samples of LMF‐EC have a coating shell thickness of about 0.5–0.8 mm.
6
Ag Nanowire-Polymer Composite Fabrication
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Ag nanowire solution (diameter: 45 nm, length: 25 μm, concentration: 1 wt%) was purchased from Kechuang New Material Co., Ltd. (Luoyang, China). Ecoflex, a type of polymer, was purchased from SMOOTH ON, INC. (Macungie, PA, USA). Conductive carbon black (diameter: 30–45 nm) was purchased from XFNANO, INC. (Nanjing, China). PVA (polyvinyl alcohol) was purchased from Shanghai Shifeng Science and Technology Co., Ltd. (Shanghai, China). The nano adhesive used in the experiment was purchased from Shenzhen Nade Adhesive Technology Co., Ltd. (Shenzhen, China). Latex gloves with a thickness of 0.12 mm were purchased from AMMEX Co., Ltd. (Kent, WA, USA).
7
Flexible Ultrasound Array Fabrication
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The Cu–PI electrode was transferred to the acrylic slide spin-coated with Ecoflex (00-30, Smooth-On) by water-soluble tape (9969B, 3M). Using ACF tape (7303, 3M), a flexible flat cable was connected to the ACF connection pad of the upper electrode. Top and bottom electrodes, 16 1–3 composite piezoelectric transducers, and two copper cylinders were then bonded together with conductive silver paste, and they were cured at 75 °C for 1 h. Next, they were encapsulated with Ecoflex under negative pressure to remove gaps. After Ecoflex was cured at room temperature for 3 h, and the acrylic slide and excess Ecoflex were peeled off. Finally, medical silicone adhesive was applied to the bottom of the flexible ultrasound array so that it can adhere tightly to the skin, eliminating gaps to avoid the use of gels.
8
NdFeB Particles in PDMS-Ecoflex Composites
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The neodymium‐iron‐boron (NdFeB) particles were purchased from Magnequench, China. Polydimethylsiloxane (PDMS) base and curing agent (Sylgard 184 kit) were purchased from Dow Corning, USA. Ecoflex (model of 00–50) was from Smooth‐On, Inc, USA. The substrate of copper coil was purchased from Chengdu Do‐itc New Material Co., Ltd. (China), and subsequently processed via laser machine for coil pattern formation.
9
Ecoflex Substrate Molding Protocol
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Two Cu molds for the top and bottom substrates were processed by machining. Ecoflex (00-30, Smooth-On) was then poured into them to undergo 3 hours of curing. The top and bottom substrates were then peeled off from the molds.
10
Ecoflex-based Conductive Nanocomposite
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MWCNTs with an average diameter of 50 nm and a length less than 10 μm were purchased from XFNANO Inc. (Nanjing, China). Ecoflex was obtained from Smooth-On, Inc. (Macungie, U.S). The conductive metal tape was brought from MaoYe Inc. (Shenzhen, China). All the materials were utilized as received without any further purification.
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