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Thioanisol

Manufactured by Merck Group
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Thioanisol is a chemical compound that serves as a precursor in the production of various pharmaceutical and agricultural products. It is a colorless or pale yellow liquid with a characteristic odor. Thioanisol is primarily used as an intermediate in the synthesis of other chemical compounds, but its specific core function does not involve interpretation or extrapolation.

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Lab products found in correlation

Phenol, by Merck Group (4 mentions) N n diisopropylethylamine, by Merck Group (4 mentions) Trifluoroacetic acid, by Merck Group (3 mentions) Methyl tert butyl ether (mtbe), by Merck Group (3 mentions) Dichloromethane, by Merck Group (3 mentions) Dimethylformamide, by Merck Group (3 mentions) Acetic anhydride, by Merck Group (3 mentions) Fmoc lys boc wang resin, by AAPPTec (3 mentions) N n diisopropylcarbodiimide, by Merck Group (3 mentions) Hydroxybenzotriazole, by Merck Group (3 mentions) O 7 azabenzotriazol 1 yl n n n n tetramethyluronium hexafluorophosphate hatu, by Merck Group (3 mentions) Acetic acid, by Merck Group (3 mentions) N trityl 1 2 ethanediamine, by Merck Group (3 mentions) Lysotracker, by Thermo Fisher Scientific (2 mentions) Iodine, by Merck Group (2 mentions) Bacmam 2, by Thermo Fisher Scientific (2 mentions) Bodipy tr ceramide, by Thermo Fisher Scientific (2 mentions) Q3 system, by Merck Group (2 mentions) Wang resin fmoc tyr tbu wang resin, by AAPPTec (2 mentions) Celllight peroxisome gfp, by Thermo Fisher Scientific (2 mentions)

4 protocols using thioanisol

1

Peptide Synthesis and Immunofluorescence Assay

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All the fluorenylmethyloxycarbonyl (Fmoc) amino acids and Fmoc-Lys (Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). Dichloromethane (DCM), acetic acid, acetic anhydride, thioanisole, phenol, hydroxybenzotriazole (HOBt), N,N-diisopropylethylamine (DIEA), N-trityl-1,2-ethanediamine, phenol, thioanisol, dimethylformamide (DMF), N,N′-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), iodine, methyl tert-butyl ether (MTBE), O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HATU), and dexamethasone (DEX) were purchased from Sigma-Aldrich (St Louis, MO). Hematoxylin and eosin (H&E) stains were purchased from MilliporeSigma (St Louis, MO). Rabbit anti-pANXA2 (phospho-Tyr24) antibody was purchased from Signalway Antibody (College Park, MD). AlexaFluor 594-conjugated donkey anti-rabbit antibody was purchased from Thermo Fisher Scientific (Waltham, MA).
Tsen S.W., Springer L.E., Sharmah Gautam K., Tang R., Liang K., Sudlow G., Kucharski A., Pham C.T, & Achilefu S. (2021). Non-invasive monitoring of arthritis treatment response via targeting of tyrosine-phosphorylated annexin A2 in chondrocytes. Arthritis Research & Therapy, 23, 265.
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2

Synthesis of Fluorescent Peptide Conjugates

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9-Fluorenylmethoxycarbonyl (Fmoc) protected amino acid derivatives and Rink amide 4-methylbenzhydrylamine (Rink amide MBHA) resin were purchased from IRIS Biotech GmbH (Marktredwitz, Germany). The reagents for synthesis were N,N’-diisopropylcabodiimide (DIC), N,N-diisopropyl-ethylamine (DIEA), 1-hydroxybenzotriazole (HOBt), 1,8-diazabicyclo-[5.4.0]undec-7-ene (DBU), piperidine, thioanisol, phenol, 1,2-ethanedithiol, 5(6)-carboxyfluorescein (Cf), and they were purchased from Sigma-Aldrich (Budapest, Hungary). Trifluoroacetic acid (TFA) and solvents (N,N-dimethylformamide (DMF) and dichloromethane) (DCM)) for synthesis, as well as HPLC grade acetonitrile, were obtained from Molar Chemicals (Halásztelek, Hungary). Cf-Penetratin was provided by Kata Horváti (MTA-ELTE Research Group of Peptide Chemistry; Eötvös Loránd University, Institute of Chemistry) [17 (link),18 (link)].
Bősze S., Zsila F., Biri-Kovács B., Szeder B., Majer Z., Hudecz F, & Uray K. (2020). Tailoring Uptake Efficacy of HSV-1 gD Tailoring Uptake Efficacy of Hsv-1 GD Derived Carrier Peptides. Biomolecules, 10(5), 721.
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3

Peptide Synthesis and Cellular Assays

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All the fluorenylmethyloxycarbonyl (Fmoc) amino acids, Wang resin Fmoc-Tyr(tBu)-Wang resin and Fmoc-Lys(Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). Dichloromethane (DCM), acetic acid, acetic anhydride, phenol, hydroxybenzotriazole (HOBt), N,N-diisopropylethylamine (DIEA), N-trityl-1,2-ethanediamine, phenol, thioanisol, dimethylformamide (DMF), N,N’-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), iodine, methyl tert-butyl ether (MTBE) and O-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU) were purchased from Sigma-Aldrich (St. Louis, MO, USA). For a full list of chemicals used and their details, please refer to Supplementary Table 1. Water was obtained from a Millipore Q3 system. Lysotracker, BODIPY TR Ceramide, CellLight Peroxisome-GFP, BacMam 2.0, and Fluo-4 calcium probe were purchased from Thermo Fisher Scientific (Waltham, MA). Chlorpromazine hydrochloride, filipin complex, and amiloride hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Reagents for organelle staining included VectaCell Rhodamine 123 (Vector Laboratories, Burlingame, CA, USA).
Shen D., Xu B., Liang K., Tang R., Sudlow G.P., Egbulefu C., Guo K., Som A., Gilson R., Maji D., Mondal S., Habimana-Griffin L., Akers W., Li S., Liu Y., Bloch S., Kurkure S., Nussinov Z., Seidel A., Tsen S.W, & Achilefu S. (2020). Selective imaging of solid tumours via the calcium-dependent high-affinity binding of a cyclic octapeptide to phosphorylated Annexin A2. Nature biomedical engineering, 4(3), 298-313.
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4

Peptide Synthesis and Cellular Assays

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All the fluorenylmethyloxycarbonyl (Fmoc) amino acids, Wang resin Fmoc-Tyr(tBu)-Wang resin and Fmoc-Lys(Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). Dichloromethane (DCM), acetic acid, acetic anhydride, phenol, hydroxybenzotriazole (HOBt), N,N-diisopropylethylamine (DIEA), N-trityl-1,2-ethanediamine, phenol, thioanisol, dimethylformamide (DMF), N,N’-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), iodine, methyl tert-butyl ether (MTBE) and O-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU) were purchased from Sigma-Aldrich (St. Louis, MO, USA). For a full list of chemicals used and their details, please refer to Supplementary Table 1. Water was obtained from a Millipore Q3 system. Lysotracker, BODIPY TR Ceramide, CellLight Peroxisome-GFP, BacMam 2.0, and Fluo-4 calcium probe were purchased from Thermo Fisher Scientific (Waltham, MA). Chlorpromazine hydrochloride, filipin complex, and amiloride hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Reagents for organelle staining included VectaCell Rhodamine 123 (Vector Laboratories, Burlingame, CA, USA).
Shen D., Xu B., Liang K., Tang R., Sudlow G.P., Egbulefu C., Guo K., Som A., Gilson R., Maji D., Mondal S., Habimana-Griffin L., Akers W., Li S., Liu Y., Bloch S., Kurkure S., Nussinov Z., Seidel A., Tsen S.W, & Achilefu S. (2020). Selective imaging of solid tumours via the calcium-dependent high-affinity binding of a cyclic octapeptide to phosphorylated Annexin A2. Nature biomedical engineering, 4(3), 298-313.
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