Vectastain universal quick hrp kit
The VECTASTAIN® Universal Quick HRP Kit is a ready-to-use system for the detection of target antigens in immunohistochemical and immunocytochemical applications. The kit includes a universal biotinylated secondary antibody and a preformed avidin-biotinylated horseradish peroxidase (HRP) complex for a sensitive and rapid detection method.
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8 protocols using vectastain universal quick hrp kit
Immunohistochemical Analysis of Oct4 in Murine Tumors
Histopathological Analysis of Tissue Samples
Immunostaining of Tumor Tissues
Quantifying SUR1 Expression in Cervical Cancer
Paraffin Immunohistochemistry Staining Protocol
Immunohistochemical Analysis of MMP-9 and TNF-α
Quantifying Phospho-JNK in Cervical Cancer Tissue
Immunohistochemistry and Immunofluorescence Protocols
For immuno uorescence staining of culture cells, 100,000 cells were seeded on coverslips in 24-well plates. Next, cells were washed with PBS and xed by 4% PFA for 10 min. Then cells were blocked with 5% BSA, 0.2% Triton X-100 in PBS for 1 hour and incubated with primary antibody in blocking buffer at 4℃ overnight. Next day, cells were incubated with secondary antibody for 2 hours in room temperature in dark, followed by incubating with DAPI for 20 min, and then coverslips were washed and mounted in antifade mounting medium. The uorescence images were acquired by using CONFOCAL microscope with a 25×or 63× objective and analyzed with ImageJ Software.
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