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Automated stainer

Manufactured by Leica
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The Automated Stainer is a compact and efficient device designed for automated histological staining procedures. It features a programmable control system that ensures consistent and reproducible staining results across multiple samples. The Automated Stainer is capable of handling a variety of staining protocols and can be seamlessly integrated into your laboratory workflow.

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Lab products found in correlation

Spectra hemalast, by Leica (2 mentions) Pro par xylene substitute, by Anatech (2 mentions) Rabbit anti e cadherin, by Abcam (1 mentions) Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions) Rabbit anti hgf, by Santa Cruz Biotechnology (1 mentions) Goat anti shh, by R&D Systems (1 mentions) Rabbit anti igf 1, by Abcam (1 mentions) Rabbit anti anxa2, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Leica Bond Rx Automated Stainer (19 citations) Bond-III automated IHC stainer (12 citations) Bond III automated IHC/ISH stainer (12 citations) BOND RX Fully Automated Research Stainer (11 citations) BOND-III automated stainer (10 citations) Bond-Max Automated IHC stainer (8 citations) BOND-MAX Automated IHC/ISH Stainer (7 citations) Bond RXm Automated Stainer (4 citations) Automated slide stainer (3 citations) Leica stainer (2 citations)

4 protocols using automated stainer

1

Immunohistochemical Staining of AnxA2 in Pancreatic Tumors

Cited 1 time
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Immunohistochemical staining for AnxA2 was performed on slides containing human pancreatic tumor tissue using a previously described protocol on an automated stainer (Leica Microsystems) [12 (link)].
Murphy A.G., Foley K., Rucki A.A., Xia T., Jaffee E.M., Huang C.Y, & Zheng L. (2017). Stromal Annexin A2 expression is predictive of decreased survival in pancreatic cancer. Oncotarget, 8(63), 106405-106414.
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2

Evaluation of AnxA2 and Its Phosphorylation

Cited 2 times
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Elution of AnxA2 (26 (link))and Western blot with rabbit anti-AnxA2, mouse anti-P-Y23-AnxA2, mouse anti-beta-actin, rabbit anti-HGF (all from Santa Cruz Biotechnology), rabbit anti-IGF-1(Abcam) or goat anti-Shh (R&D Systems) antibodies were described previously (12 (link)) Immunohistochemistry (IHC) staining for E-Cadherin and HGF was performed with rabbit anti-E-Cadherin (Abcam) and rabbit anti-HGF (Santa Cruz Biotechnology) antibodies using a standard protocol on an automated stainer from Leica Microsystems. IHC for SMA was performed as previously described (27 (link)). IHC for AnxA2 was conducted with mouse anti-AnxA2(Invitrogen), or mouse anti-P-Y23-AnxA2 antibodies, IHC for Shh with goat-anti-Shh (R&D), and IHC for IGF-1 with rabbit-anti-IGF-1(Abcam) antibodies as described previously (13 (link)). All IHC slides were analyzed and scored by a pathologist (A.L). The levels of secreted IGF-1 were measured by ELISA (R&D) following the manufacturer’s instructions.
Rucki A.A., Foley K., Zhang P., Xiao Q., Kleponis J., Wu A.A., Sharma R., Mo G., Liu A., Van Eyk J., Jaffee E.M, & Zheng L. (2016). Heterogeneous stromal signaling within the tumor microenvironment controls the metastasis of pancreatic cancer. Cancer research, 77(1), 41-52.
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3

Automated Histological Staining Procedure

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The staining procedure was run on a Leica automated stainer. Slides were heated to 65°C for 9 minutes, then cleared [with Pro-Par xylene substitute (Anatech Ltd)], and rehydrated before treatment with SPECTRA Hemalast (Leica) and sequential staining with hematoxylin for 4 minutes, bluing for 30 seconds, and eosin for 1 minute following a 1-minute 80% ethyl alcohol wash. Slides were then dehydrated, recleared with Pro-Par, and cover slipped using Permount.
Boudreau C.E., Najem H., Ott M., Horbinski C., Fang D., DeRay C.M., Levine J.M., Curran M.A, & Heimberger A.B. (2021). Intratumoral Delivery of STING Agonist Results in Clinical Responses in Canine Glioblastoma. Clinical Cancer Research, 27(20), 5528-5535.
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4

Automated Histological Staining Procedure

Check if the same lab product or an alternative is used in the 5 most similar protocols
The staining procedure was run on a Leica automated stainer. Slides were heated to 65degC for 9 minutes, then cleared (with Pro-Par xylene substitute (Anatech Ltd)), and rehydrated the slides before treatment with SPECTRA Hemalast (Leica) and sequential staining with hematoxylin for 4 min, bluing for 30 sec, and eosin for 1 min following a 1 min 80% ethyl alcohol wash. Slides were then dehydrated, re-cleared with Pro- Par, and cover slipped using Permount.
Boudreau C.E., Najem H., Ott M., Horbinski C., Fang D., DeRay C.M., Levine J.M., Curran M.A, & Heimberger A.B. (2021). Intratumoral Delivery of STING Agonist Results in Clinical Responses in Canine Glioblastoma. Clinical Cancer Research, 27(20), 5528-5535.
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