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Elisa microtiter plate autoreader

Manufactured by Molecular Devices
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Sourced in United States

The ELISA microtiter plate autoreader is a laboratory instrument designed to automate the process of reading and analyzing enzyme-linked immunosorbent assay (ELISA) plates. It measures the absorbance of light at specific wavelengths, providing quantitative data on the levels of target analytes in the samples.

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Lab products found in correlation

Il 17, by BioLegend (1 mentions) Mouse ifn γ, by BioLegend (1 mentions) Tnf α, by BioLegend (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions)

2 protocols using elisa microtiter plate autoreader

1

Cytokine Profiling in Mouse Serum and Cell Culture

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ELISA was performed according to the manufacturer's instructions (R&D Systems). Human IL-17, IFN-γ, transforming growth factor (TGF)-β, IL-10, and vascular endothelial growth factor (VEGF) were measured in the cell culture supernatant. Whole blood collected from mouse was centrifuged at 400 × g for 10 min, and the serum was analyzed for the levels of human IL-10, TGF-β, tumor necrosis factor (TNF)-α, and IL-6. The plates were then read using an ELISA microtiter plate autoreader at 450 nm (Molecular Devices, Sunnyvale, CA, United States).
Park M.Y., Lim B.G., Kim S.Y., Sohn H.J., Kim S, & Kim T.G. (2019). GM-CSF Promotes the Expansion and Differentiation of Cord Blood Myeloid-Derived Suppressor Cells, Which Attenuate Xenogeneic Graft-vs.-Host Disease. Frontiers in Immunology, 10, 183.
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2

Serum Cytokine Analysis in Immunized Mice

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After euthanasia using a CO2 chamber at 3 weeks after immunization, the serum in whole blood by cardiac puncture was collected for mouse IFN-γ (Cat# 430807, BioLegend, San Diego, CA, USA), IL-17 (Cat# 432507, BioLegend), TNF-α (Cat# 430907, BioLegend), and IL-1β (Cat# BMS6002, Invitrogen) analyses. ELISA kits were analyzed from accordance with the directions provided by the manufacturer. The samples were then analyzed with an ELISA microtiter plate auto reader at 450 nm (Molecular Devices, San Jose, CA, USA).
Lee J.Y., Kim S., Sohn H.J., Kim C.H., Kim T.G, & Lee H.S. (2023). Local Myeloid-Derived Suppressor Cells Impair Progression of Experimental Autoimmune Uveitis by Alleviating Oxidative Stress and Inflammation. Investigative Ophthalmology & Visual Science, 64(13), 39.
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