The purified HEV VLP and immune complexes were diluted in PBS (pH 7.4) and then absorbed onto 200 mesh carbon-coated copper grids (Quantifoil Micro Tools) for 1 min. The grids were washed twice with double-distilled water and negatively stained with 2% phosphotungstic acid (pH 6.4) for 30 s. Specimens were evaluated and imaged with the FEI Tecnai T12 TEM at 25,000× magnification.
200 mesh carbon coated copper grids
Manufactured by Quantifoil
Sourced in United States
200-mesh carbon-coated copper grids are a type of laboratory equipment used in electron microscopy. They provide a stable and conductive support for samples being examined under an electron microscope. The grids have a mesh size of 200, and the copper surface is coated with a thin layer of carbon for improved conductivity and sample adhesion.
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Lab products found in correlation
3 protocols using 200 mesh carbon coated copper grids
1
Negative Staining of HEV VLPs
2
Negative Staining Electron Microscopy of gB Proteins
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The gB proteins were analyzed by negative staining electron microscopy as previously reported (49 (link)). Briefly, diluted protein samples were applied to 200-mesh carbon-coated copper grids (Quantifoil) for 5 min and excess solution was removed. After two washes with doubly distilled water, grids were immediately negatively stained for 30 s with freshly filtered 2% phosphotungstic acid (pH 6.4). Grids were examined with a FEI Tecnai T12 TEM (FEI) at an accelerating voltage of 120 kV and photographed at a magnification of 25,000-fold.
3
Negative Staining Electron Microscopy of VLPs
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The VLPs were analyzed by negative staining electron microscopy as previously reported [24 (link)]. Briefly, diluted unmodified HBc149 and chimeric HBc149 samples were applied to 200-mesh carbon-coated copper grids (Quantifoil, PA, USA) for 5 min and excessive solution were removed. After two washes with double distilled water, grids were immediately negatively stained for 30 s with freshly filtered 2% phosphotungstic acid (pH 6.4). Grids were examined with a FEI Tecnai T12 TEM (FEI, Portland, OR, USA) at an accelerating voltage of 120 kV and photographed at a magnification of 25,000-fold.
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