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GDF15 is a lab equipment product offered by Cell Signaling Technology. It is a recombinant protein that represents the mature form of the Growth Differentiation Factor 15 (GDF15) protein. GDF15 is a member of the transforming growth factor-beta (TGF-β) superfamily, which plays a role in various cellular processes.

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Lab products found in correlation

Iscript cdna synthesis reagent, by Bio-Rad (1 mentions) Phospho c jun ser63, by Cell Signaling Technology (1 mentions) Antibody to β actin, by Merck Group (1 mentions) C myc, by Cell Signaling Technology (1 mentions) Pdgfrβ, by Cell Signaling Technology (1 mentions) Pdgfrα, by Cell Signaling Technology (1 mentions) Cyr61, by Cell Signaling Technology (1 mentions) Ddit3, by Cell Signaling Technology (1 mentions) Runx1, by Cell Signaling Technology (1 mentions) Recombinant human pdgf bb, by R&D Systems (1 mentions) C jun, by Cell Signaling Technology (1 mentions) Sds page gel, by Bio-Rad (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Sf3b2, by Santa Cruz Biotechnology (1 mentions) Anti grb2, by BD (1 mentions) Protease and phosphatase inhibitor cocktail, by Merck Group (1 mentions) Gdf15 antibody, by R&D Systems (1 mentions)

2 protocols using gdf15

1

Profiling PDGF-BB Signaling Pathways

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Recombinant human PDGF-BB was from R&D Systems (Minneapolis, MN). Antibodies to PDGFRα, PDGFRβ, phospho-PDGFRα/β Tyr849/Tyr857, c-Jun, phospho-c-Jun Ser63, c-Myc, EGR1, RUNX1, DDIT3, CYR61 and GDF15 were from Cell Signaling Technology (Danvers, MA); antibodies to Myb and NFAT5 were from Epitomics (Burlingame, CA); antibodies to SOX5 and GAPDH were from Santa Cruz Biotechnology (Santa Cruz, CA); antibody to β-actin was from Sigma Aldrich (Sigma Chemical Company, St. Louis, MO); antibody to DIAPH3 was a generous gift from Henry Higgs, Dartmouth Medical School. The c-Myc TF ELISA kit was from Active Motif (Carlsbad, CA). SP600125 and 10048-F4 were from EMD Biosciences (Billerica, MA). iScript cDNA synthesis reagents were from BioRad Laboratories (Hercules, CA). Universal PCR master mix for qRT-PCR and gene-specific assays were from Applied Biosystems (now Life Technologies, Grand Island, NY). Primers for human transcripts were as follows: Hs00171022_m1 for CXCL12; Hs00998500_g1 for CYR61; Hs01107330_m1 for DIAPH3; Hs02758991_g1 for GAPDH; Hs00171132_m1 for GDF15; Hs01110250_m1 for HMOX-1; Hs00998018_m1 for PDGFRA; and Hs01019589_m1 for PDGFRB. Primers for mouse transcripts were Mm00487499_g1 for CYR61; Mm99999915_g1 for GAPDH; Mm00442228_m1 for GDF15; Mm00435546_m1 for Pdgfrb.
Yang W., Ramachandran A., You S., Jeong H., Morley S., Mulone M.D., Logvinenko T., Kim J., Hwang D., Freeman M.R, & Adam R.M. (2014). Integration of proteomic and transcriptomic profiles identifies a novel PDGF-MYC network in human smooth muscle cells. Cell Communication and Signaling : CCS, 12, 44.
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2

Western Blot for Protein Analysis

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Example 3

Western Blot Analysis: Cells were collected by gently scraping in the presence of PBS (Gibco, Life Technologies), followed by centrifugation and disruption of the cell pellet in lysis buffer (50 mM HEPES, pH 7.5, 150 mM NaCl, 1.5 mM MgCl2, 1 mM EGTA, pH 8.4, 10% glycerol, 1% Triton X-100, 1 mM PMSF, 1 mM sodium orthovanadate (NA3VO4), Phosphatase and Protease Inhibitor Cocktails (Sigma). Whole-cell lysates (30 to 70 μg) were separated on a 4-15% SDS-PAGE gel (Bio-Rad, Hercules, Calif.). GDF15 and E2F-1 antibodies were purchased from Cell Signaling Technology (Beverly, Mass.). The antibody to detect p63RhoGEF was obtained from GeneTex (Irvine, Calif.). GDF15 antibody was purchased from R&D Systems (Minneapolis, Minn.), and anti-GRB2 was from BD Transduction Laboratories (San Jose, Calif.). SF3B2 and 14-3-3ζ antibodies were obtained from Santa Cruz Biotechnology.

US10308939B2. Use of an miRNA to reduce proliferation of a cancer cell (2019-06-04). Board of Supervisors of Louisiana State University and Agricultural and Mechanical College [US]. Inventors: Francesca Peruzzi [US], Duane Jeansonne [US].
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