Hlepics

Manufactured by Thermo Fisher Scientific

Sourced in United States

The HLEpiCs is a lab equipment product designed for cell culture applications. It is a multi-well cell culture system used for the study of human and animal epithelial cells.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Hlepics, by American Type Culture Collection (2 mentions) Site directed mutagenesis kit, by Vazyme (1 mentions)

2 protocols using hlepics

Transfection of Human Lens Epithelial Cells

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Control human lens epithelia cells (HLEpiCs) from an unaffected subject were obtained from American Type Culture Collection (ATCC) and cultivated in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA). The cells were cultured at 37 °C in a humidified 5% CO₂ atmosphere.
Recombinant human GJA8 was amplified from genomic DNA and inserted into the pEGFP-N1 vector. The mutant GJA8 gene was obtained using a Site-Directed Mutagenesis Kit (Vazyme, China). Recombinant plasmids containing the normal or mutant gene were transfected into HLEpiCs using Lipofectamine 2000 (Invitrogen, USA) according to the manufacturer’s protocols.

Li L., Fan D.B., Zhao Y.T., Li Y., Yang Z.B, & Zheng G.Y. (2019). GJA8 missense mutation disrupts hemichannels and induces cell apoptosis in human lens epithelial cells. Scientific Reports, 9, 19157.

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Orai3 Regulation in Foam Cell Formation

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The HLEpiCs were purchased from the American Type Culture Collection (HB-8065, Manassas, VA, USA) and then cultured in Dulbecco's modi ed Eagle's medium (DMEM; Gibco) containing 10% fetal bovine serum (FBS;Gibco) at 37 °C in an incubator with 5% carbon dioxide. The culture media in the oxLDL groups contained various concentrations of oxLDL(Guangzhou Yiyuan biotech.Co.,Ltd.), whereas media for the control groups contained equal volumes of DMEM. The HLEpiCs were transfected with siRNA speci c to human Orai3 (5′-GGGUCAAGUUUGUGCCCAU-3′) or with scrambled siRNA (5′-ACGCGUAACGCGGGAAUUU-3′) using Lipofectamine 2000 (Invitrogen) by following the manufacturer's instructions. The siRNAs were designed and purchased from Biomics Biotechnologies. The nal siRNA concentration was 200 nm/L. The HLEpiCs were cultured in an incubator with 5% carbon dioxide at 37 °C for 24 h. Western blotting was conducted to determine the transfection e ciency.

Zhang R., Bai S., Yang F., Zhou Z., Chen L., Du J., Shen B., & Wang Y. (2020). Oxidized low-density lipoprotein enhances Orai3 expression levels to increase proliferation of human lens epithelial cells.

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