BV2 cells (1 × 105 cells per well) were transfected with shRNA plasmids targeting murine AnxA1 from the MISSION TRC shRNA collection (Sigma-Aldrich, St. Louis, MO, USA), or with an empty plasmid control (termed pKCON). Cells were transfected for 48 h using FuGENE HD (Promega, Madison, Wisconsin, USA) according to the manufacturer's instructions, followed by selection for stable clones using puromycin (Promega, Madison, Wisconsin, USA). Transfection was confirmed by western blot analysis.
Mission trc shrna collection
Manufactured by Merck Group
Sourced in United States
The MISSION TRC shRNA collection is a comprehensive library of short hairpin RNA (shRNA) molecules designed for gene knockdown studies. The collection targets a wide range of human and mouse genes, providing researchers with a tool to investigate gene function and explore potential therapeutic targets.
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Lab products found in correlation
2 protocols using mission trc shrna collection
1
Knockdown of murine AnxA1 in BV2 cells
2
Knockdown of ANXA1, TSPO, and TLR4 in BV-2 Cells
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The BV-2 cells (1 x 10 5 cells per well) were transfected with the anti-sense cDNA plasmid to ANXA1 containing 476 bp namely pRc/CMV ANXA1 AS [30] ,
and it was used to knockdown ANXA1 expression in BV-2 cells (ANXA1 -/-).
shRNA plasmids from the MISSION TRC shRNA collection (Sigma-Aldrich, St.
Louis, MO, USA) targeting TSPO (shRNA-TSPO -TSPO -/-) or TLR4 (shRNA-TLR4 -TLR4 -/-) receptors were used to knockdown the expression of each receptors respectively. BV-2 cells were transfected using FuGENE HD (Promega, Madison, Wisconsin, EUA) according to the manufacturer's instructions. After 48 hours transfection, cells were selected using geneticin (G-418, Promega, Madison, Wisconsin, EUA) for pRC/CMV plasmid and puromycin (Sigma-Aldrich, Darmstadt, Germany) for MISSIONs shRNA.
and it was used to knockdown ANXA1 expression in BV-2 cells (ANXA1 -/-).
shRNA plasmids from the MISSION TRC shRNA collection (Sigma-Aldrich, St.
Louis, MO, USA) targeting TSPO (shRNA-TSPO -TSPO -/-) or TLR4 (shRNA-TLR4 -TLR4 -/-) receptors were used to knockdown the expression of each receptors respectively. BV-2 cells were transfected using FuGENE HD (Promega, Madison, Wisconsin, EUA) according to the manufacturer's instructions. After 48 hours transfection, cells were selected using geneticin (G-418, Promega, Madison, Wisconsin, EUA) for pRC/CMV plasmid and puromycin (Sigma-Aldrich, Darmstadt, Germany) for MISSIONs shRNA.
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