A549 and H661 cells grown on glass slides were rinsed with PBS and fixed in 4% formaldehyde at room temperature for 20 min. The cells were washed 3 times with PBS and processed for indirect immunofluorescence as previously described [42 (link)]. The slides were incubated with the mouse anti-acetylated-α-tubulin antibody, rabbit anti-HDAC6, anti-His (1:400, Santa Cruz biotechnology, Inc.), and anti-Flag antibody (1:400, Sigma). The cells were then washed and incubated with FITC-labeled goat anti-rabbit or TR-labeled goat anti-mouse (1:400, Santa Cruz biotechnology, Inc.) antibody. Images were taken with an Olympus fluorescence microscope.
Tr labeled goat anti mouse
Manufactured by Santa Cruz Biotechnology
Sourced in United States
TR-labeled goat anti-mouse is a secondary antibody conjugated with Texas Red (TR) fluorescent dye. It is designed to bind to mouse primary antibodies, allowing for the detection and visualization of target proteins in various immunoassays and imaging applications.
Automatically generated - may contain errors
Lab products found in correlation
Fitc labeled goat anti rabbit, by Santa Cruz Biotechnology (2 mentions)
Fluorescence microscope, by Olympus (1 mentions)
Anti flag antibody, by Merck Group (1 mentions)
U lh100hg, by Olympus (1 mentions)
Sc 57535, by Santa Cruz Biotechnology (1 mentions)
Sc 11420, by Santa Cruz Biotechnology (1 mentions)
2 protocols using tr labeled goat anti mouse
1
Immunofluorescence Staining of Acetylated-α-Tubulin, HDAC6, His, and Flag
2
Immunofluorescence Assay for β-Catenin and HDAC6
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The cells grown on glass slides were rinsed with PBS and fixed in 4% formaldehyde at room temperature for 20 mins. The cells were washed three times with PBS and processed for indirect immunofluorescence as previously described [30] (link). The slides were incubated with the mouse anti-β-catenin antibody and rabbit anti-HDAC6 (sc-57535 and sc-11420, 1:400, Santa Cruz Biotechnology Inc., CA, USA). The cells were then washed and incubated with FITC-labeled goat anti-rabbit or TR-labeled goat anti-mouse (1:400, Santa Cruz Biotechnology Inc., CA, USA) antibody. Images were taken with an Olympus fluorescence microscope (U-LH100HG, Olympus Corporation, Japan).
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