Monoclonal anti-Rab5 (sc46692) and anti-RhoA (sc-418) were from Santa Cruz Biotechnologies. Other antibodies included monoclonal anti-ALS2 (ab170896, Abcam), anti-HIF1α (#610959, BD Transduction Laboratories) and ChIP-grade polyclonal anti-HIF-1α (ab2185, Abcam). Goat anti-rabbit and goat anti-mouse antibodies coupled to horseradish peroxidase and anti-actin antibody (#A5316, SIGMA) were from Bio-Rad Laboratories (Hercules, CA). Tissue culture medium, antibiotics and fetal bovine serum were from GIBCO Life Technologies (Grand Island, NY) and HyClone Laboratories (Logan, UT). Glutathione-Sepharose 4B was from GE Healthcare (Piscataway, NJ). The EZ-ECL chemiluminescent substrate was from Pierce Chemical (Rockford, IL). The HIF-1α inhibitor (10uM, sc-205346) and siRNA constructs targeting HIF-1α (mix of 3 different siRNAs targeting human HIF-1α, sc-35561), ALS2 (mix of 3 different siRNAs targeting human ALS2, sc-60154) and siRNA control (sc-37007), were from Santa Cruz Biotechnology. Transfections were performed with Optimem (#31985088, Gibco), the Lipofectamine 2000 (#11668027) and the Lipofectamine RNAiMAX (13778075), both from Invitrogen.
Sc 35561
Manufactured by Santa Cruz Biotechnology
Sc-35561 is a laboratory product manufactured by Santa Cruz Biotechnology. It is a piece of equipment designed for use in scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Ab2185, by Abcam (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Anti rhoa sc 418, by Santa Cruz Biotechnology (1 mentions)
Tissue culture medium, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Sc 37007, by Santa Cruz Biotechnology (1 mentions)
Glutathione sepharose 4b, by GE Healthcare (1 mentions)
Ez ecl chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
2 protocols using sc 35561
1
Investigating HIF-1α and ALS2 Signaling
2
Regulation of HIF-1α by miRNA-145 and siRNA Knockdown
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The recombinant vector pc-DNA TM 3.1 (+) -HIF-1a with full-length of HIF-1a cording sequences or empty vector as its negative control (Thermo Fisher Scientific) were transfected into cells, respectively. The siRNA against HIF-1a was purchased from Santa Cruz Biotechnology (sc-35561, Santa Cruz, CA). HIF-1a antibody (C-19) (sc-8711, Santa Cruz, CA) which was confirmed as no silencing double-stranded RNA, was used as a control for siRNA experiments. SGK1 silenced by SGK1 siRNA (sc-33774, Santa Cruz, CA) was performed by the same experimental protocol [21] (link). For miRNA transfection, cells were transfected with miR-145 mimic, miR-145 inhibitor and negative miRNA control (scramble) (GenePharma, Shanghai, China). All the transfections were performed by using Lipofectamine 3000 (Invitrogen Life Technologies, Carlsbad, CA, USA) according to the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!