Iron and zinc contents were determined by AOAC (2019 ) method 999.10 and measured by Inductively Coupled Plasma Atomic Emission Spectrometry (Thermo iCAP‐6500 DV; Thermo Fisher Scientific, Waltham, MA, USA) following the validated protocol of the Chemisch Biologisch Laboratorium Bodem (CBLB), Wageningen, the Netherlands.
Thermo icap 6500 dv
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Thermo iCAP‐6500 DV is an inductively coupled plasma optical emission spectrometer (ICP-OES) instrument designed for elemental analysis. It features dual-view technology, allowing for both axial and radial plasma observation to provide a wide linear dynamic range and superior sensitivity. The instrument is capable of detecting and quantifying a wide range of elements in various sample matrices.
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2 protocols using thermo icap 6500 dv
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Analytical Determination of Iron and Zinc
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ICP-AES Analysis of Fe and Zn
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Fe and Zn were measured by ICP-AES (inductively coupled plasma-atomic emission spectrometry) (Thermo iCAP-6500 DV; Thermo Fisher Scientific, Waltham, USA), following the validated Chemisch Biologisch Laboratorium Bodem (CBLB, Wageningen, the Netherlands) protocol. Briefly, 300 mg of ground sample was digested with concentrated nitric-hydrochloric acid mixture, and hydrogen-peroxide in a microwave digestion system (MarsXpress; CEM Corporation, Matthews, USA), consecutively. After settling of the undissolved silica particles, the supernatant was analysed on ICP-AES. Prior to analysis, samples were dried at 70 °C overnight and ground to 0.425 mm.
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