Phospho yap1 ser127

Manufactured by Cell Signaling Technology

Phospho‐YAP1 (Ser127) is an antibody that detects the phosphorylation of YAP1 at serine 127. YAP1 is a transcriptional co-activator that plays a role in the Hippo signaling pathway, which regulates cell proliferation and apoptosis.

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Lab products found in correlation

Phospho p53 ser15, by Cell Signaling Technology (1 mentions) Slc7a11, by Cell Signaling Technology (1 mentions) Acsl4, by Abcam (1 mentions) β actin, by Merck Group (1 mentions)

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Phospho-YAP1 (5 citations)

2 protocols using phospho yap1 ser127

Protein Expression Analysis Protocol

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The proteins were isolated and separated by SDS‐PAGE. Primary antibodies against CYGB (#60228, Proteintech), YAP1 (#14074, Cell Signaling Technology), phospho‐YAP1 (Ser127) (#13008, Cell Signaling Technology), p53 (#2524, Cell Signaling Technology), phospho‐p53 (Ser15) (#9284, Cell Signaling Technology), SLC7A11 (#12691, Cell Signaling Technology), ACSL4 (#ab155282, Abcam) and GAPDH (#AP0063, Bioworld Antibodies) were used.

Ye S., Xu M., Zhu T., Chen J., Shi S., Jiang H., Zheng Q., Liao Q., Ding X, & Xi Y. (2021). Cytoglobin promotes sensitivity to ferroptosis by regulating p53‐YAP1 axis in colon cancer cells. Journal of Cellular and Molecular Medicine, 25(7), 3300-3311.

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Western Blot Protein Expression Analysis

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Western blot experiments were conducted as described previously (14 ). The following primary antibodies were used to determine protein expression by Western blot: FLAG (1:5000, Sigma F7425), YAP1 (1:1000, Cell Signaling, #4912), phospho YAP1 (Ser127) (1:1000, Cell Signaling, #4911), and β-actin (1:20000, Sigma, #A5441).The following secondary antibodies were used: Anti-Rabbit HRP (1:2000, DAKO, P0260), Anti-Mouse HRP (1:2000, DAKO, P0448) and Anti-mouse IRDye 680nm (1:5000, Li-COR 926-32222).

Schipper K., Drenth A.P., van der Burg E., Cornelissen S.P., Klarenbeek S., Nethe M, & Jonkers J. (2020). Truncated ASPP2 drives initiation and progression of invasive lobular carcinoma via distinct mechanisms. Cancer research, 80(7), 1486-1497.

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