Dissections of individual decidua were performed each in their own fresh dish of OptiMEM, and then the yolk sac and any extraembryonic tissues destined for genotyping were rinsed in separate wells of PBS in a 24-well plate. Embryos were imaged fresh in a plate of 5% FBS Opti-MEM® I Reduced Serum Medium (Life Technologies) under a Leica S9i stereo microscope with V4.12.0 Leica Application Suite software (Leica Microsystems Co.). Thereafter, the embryos were rinsed in ice-cold sterile PBS and fixed in 4% paraformaldehyde + 0.25% glutaraldehyde at 4 °C for 3 h. Fixed embryos were then dehydrated in methanol washes and stored at − 20 °C.
V4.12.0 application suite software
Manufactured by Leica
The Leica Application Suite software V4.12.0 is a core imaging software solution for Leica microscopy systems. It provides essential functionality for image acquisition, processing, and analysis.
Automatically generated - may contain errors
3 protocols using v4.12.0 application suite software
1
Embryo Dissection and Fixation Workflow
2
Embryo and Yolk Sac Isolation
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Conceptuses were isolated at E10.5 within their individual decidua, and placed in separate fresh dishes of OptiMEM (Invitrogen) for dissection. Embryos and their corresponding visceral yolk sacs were dissected apart and kept as individual samples for genotype analysis. Imaging of embryos was performed using a Leica S9i stereo microscope, and documented using V4.12.0 Leica Application Suite software (Leica Microsystems Co.). Samples were rinsed in 500μl sterile PBS and stored in 100μl PBS at −20°C until genomic DNA isolation.
3
Embryo and Yolk Sac Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Conceptuses were isolated at E10.5 within their individual decidua, and placed in separate fresh dishes of OptiMEM (Invitrogen) for dissection. Embryos and their corresponding visceral yolk sacs were dissected apart and kept as individual samples for genotype analysis. Imaging of embryos was performed using a Leica S9i stereo microscope, and documented using V4.12.0 Leica Application Suite software (Leica Microsystems Co.). Samples were rinsed in 500μl sterile PBS and stored in 100μl PBS at −20°C until genomic DNA isolation.
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