PFA-fixed and decalcified tissues were dipped in 30% sucrose solution overnight at 4°C, embedded in O.C.T. compound, and quickly frozen in liquid nitrogen. Fifteen-micrometer-thick frozen sections were mounted on poly-L-lysine coated glass slides, incubated with the rat anti-GP2 antibody (1 μg/ml) overnight, and subsequently reacted with goat anti-rat IgG covalently linked with 1 nm gold particles (1:200 dilution; Nanoprobes). Following silver enhancement with a kit (HQ silverTM; Nanoprobes), the sections were osmicated, dehydrated, and directly embedded in Epon (Nisshin EM). Ultrathin sections were prepared and stained with uranyl acetate and lead citrate for observations under an electron microscope (H-7100; Hitachi).
Goat anti rat igg
Manufactured by Nanoprobes
Sourced in Japan
Goat anti-rat IgG is a laboratory reagent used to detect and measure the presence of rat immunoglobulin G (IgG) in various biological samples. It is a secondary antibody that binds to rat IgG, allowing for its identification and quantification through various immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using goat anti rat igg
1
Ultrastructural Localization of GP2
2
Immunogold Labeling for Electron Microscopy
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Electron microscopic observation was carried out by the silver-intensified immunogold method. Cryostat sections from the cell pellets were incubated with anti-CD63 or anti-ZnT2 antibodies, followed by an incubation with goat anti-rat IgG or anti-rabbit IgG covalently linked 1-nm gold particles (1:200; Nanoprobes, Yaphank, NK). After silver enhancement using a kit (HQ silver; Nanoprobes), the sections were osmificated, dehydrated, and directly embedded in Epon (Nisshin EM, Tokyo, Japan). Ultrathin sections were prepared and stained with both uranyl acetate and lead citrate for observation under an electron microscope (H-7100; Hitachi, Tokyo, Japan).
3
Ultrastructural Localization of GP2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PFA-fixed and decalcified tissues were dipped in 30% sucrose solution overnight at 4˚C, embedded in OCT compound, and quickly frozen in liquid nitrogen. Fifteen-micrometer-thick frozen sections were mounted on poly-L-lysine coated glass slides, incubated with the rat anti-GP2 antibody (1 µg/ml) overnight, and subsequently reacted with goat anti-rat IgG covalently linked with 1 nm gold particles (1:200 dilution; Nanoprobes, Yaphank, NY).
Following silver enhancement with a kit (HQ silver; Nanoprobes), the sections were osmicated, dehydrated, and directly embedded in Epon (Nisshin EM, Tokyo, Japan). Ultrathin sections were prepared and stained with uranyl acetate and lead citrate for observations under an electron microscope (H-7100; Hitachi, Tokyo, Japan).
Following silver enhancement with a kit (HQ silver; Nanoprobes), the sections were osmicated, dehydrated, and directly embedded in Epon (Nisshin EM, Tokyo, Japan). Ultrathin sections were prepared and stained with uranyl acetate and lead citrate for observations under an electron microscope (H-7100; Hitachi, Tokyo, Japan).
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