De oiled lecithin from soy

Cold-pressed rapeseed press cake (RPC) (Brassica napus L.) was a kind gift from Gunnarshögs Jordbruk AB (Hammenhög, Sweden) and was used in all trials. No solvents were used during the oil extraction and the oil temperature did not exceed 35 °C. The dry matter of the rapeseed press cake was 91% (AOAC 2007) and the protein content on a dry basis was 31% using the Dumas method. The RPC was stored at −18 °C before the onset of the trials.
Citric acid, DL-Dithiothreitol (DTT), (S)-2-Aminobutane-1,4-dithiol hydrochloride (DTBA), and sodium hydroxide were purchased from Merck (Darmstadt, Germany). The protein 230 kit for the protein profile analysis, including all reagents, was purchased from Agilent Technologies (Agilent, Santa Clara, CA, USA). Miglyol 812 was purchased from Sasol (Witten, Germany). De-oiled lecithin from soy was purchased from Cargill (Minneapolis, MN, USA). All other chemicals were of analytical grade.

Cold-pressed rapeseed press cake (RPC) (Brassica napus L.) was a kind gift from Gunnarshögs Jordbruk AB (Hammenhög, Sweden). The rapeseeds were cold-pressed at Gunnarshögs Jordbruk AB’s production site and the oil temperature during pressing did not exceed 35 °C and no solvents were used. The hot-pressed rapeseed meal (RM) and the solvent-extracted RM was a kind gift from AAK AB (Karlshamn, Sweden). Hot pressing was typically performed at 80 °C to 100 °C. For the solvent-extracted meal, the oil residues were recovered by hexane post hot pressing. Citric acid (C₆H₈O₇, CAS 77-92-9), sodium chloride (NaCl, CAS 7647-14-5), sodium dihydrogen phosphate monohydrate (H₂Na PO₄·H₂O, CAS 7558-80-7), disodium hydrogen phosphate dodecahydrate (Na₂HPO₄·12H₂O, CAS 10039-32-4), and sodium hydroxide (NaOH, CAS 1310-73-2) were purchased from Merck (Darmstadt, Germany). Miglyol 812 was purchased from Sasol AG (Witten, Germany). Bovine serum albumin (BSA) and chicken egg white albumin were purchased from Sigma-Aldrich (St. Louis, MA, USA) and deoiled lecithin from soy was purchased from Cargill (Minneapolis, MN, USA). All other chemicals were of analytical grade.

Industrially cold-pressed rapeseed press-cake (B. napus) was a kind gift from Gunnarshögs Jordbruk AB, Hammenhög, Sweden, a producer of cold-pressed rapeseed oil. The oil temperature during extraction did not exceed 35 °C and no solvents were used during extraction. Citric acid (C₆H₈O₇, CAS 77-92-9) was purchased from Merck (Darmstadt, Germany). Miglyol 812 was purchased from Sasol (Witten, Germany). De-oiled lecithin from soy was purchased from Cargill (Minneapolis, MN, USA). All other chemicals were of analytical grade.