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Degrasyn wp1130

Manufactured by Bio-Techne
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Degrasyn/WP1130 is a small molecule inhibitor that targets the deubiquitinase USP9X. It is designed for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Hbx 41 108, by Bio-Techne (2 mentions) Ubiquitin monomer, by Merck Group (2 mentions) Pimozide, by Bio-Techne (2 mentions) Maldi tof ms materials, by Bruker (2 mentions) L434078, by Merck Group (2 mentions) Dithiothreitol (dtt), by Merck Group (2 mentions) Pr 619, by Merck Group (2 mentions) P22077, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

2 protocols using degrasyn wp1130

1

Diubiquitin Topoisomer Characterization

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Ubiquitin monomer, BSA, Tris and DTT were purchased from Sigma-Aldrich. Diubiquitin topoisomers (M1, K6, K11, K27, K29, K33, K48 and K63-linked) were purchased from Boston Biochem (Boston, MA), additional K27 diubiquitin was produced in-house57 (link)58 , whereas all MALDI-TOF MS materials (targets, matrix and protein calibration mixture) were purchased from Bruker Daltonics (Bremen, Germany). PR-619 and P22077 (Calbiochem/Merck, Darmstadt, Germany) as well as HBX 41,108, pimozide and degrasyn/WP1130 (Tocris Bioscience, Bristol, UK) and L434078 (Sigma-Aldrich, St Louis, MO, USA) were purchased commercially. Febuxostat, SJB3-019A, compound 16, NSC 697923 and BAY 11-7082 were synthesized (Supplementary Table 3).
Ritorto M.S., Ewan R., Perez-Oliva A.B., Knebel A., Buhrlage S.J., Wightman M., Kelly S.M., Wood N.T., Virdee S., Gray N.S., Morrice N.A., Alessi D.R, & Trost M. (2014). Screening of DUB activity and specificity by MALDI-TOF mass spectrometry. Nature Communications, 5, 4763.
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2

Quantitative Analysis of Diubiquitin Linkages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ubiquitin monomer, Bovine Serum Albumin (BSA), Tris and DTT were purchased from Sigma Aldrich. Diubiquitin topoisomers (M1, K6, K11, K27, K29, K33, K48 and K63-linked) were purchased from Boston Biochem (Boston, MA), additional K27 diubiquitin was produced in-house57 (link), 58 (link), whereas all MALDI-TOF MS materials (targets, matrix and protein calibration mixture) were purchased from Bruker Daltonics (Bremen, Germany). PR-619 and P22077 (Calbiochem/Merck, Darmstadt, Germany) as well as HBX 41,108, Pimozide and Degrasyn/WP1130 (Tocris Bioscience, Bristol, UK) and L434078 (Sigma-Aldrich, St. Louis, MO, USA) were purchased commercially. Febuxostat, SJB3-019A, Compound 16, NSC 697923 and BAY 11-7082 were synthesized (Supplementary Table 3).
Ritorto M.S., Ewan R., Perez-Oliva A.B., Knebel A., Buhrlage S.J., Wightman M., Kelly S.M., Wood N.T., Virdee S., Gray N.S., Morrice N.A., Alessi D.R, & Trost M. (2014). Screening of DUB activity and specificity by MALDI-TOF mass spectrometry. Nature Communications, 5, 4763.
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