The confluent monolayer cultures of GMSCs were induced with adipogenic induction medium for 3 days, followed by incubation in maintenance medium for 1 day (Adipogenic Differentiation Kit, Lonza, Switzerland). After seven cycles of induction and maintenance, the cells were washed with PBS and fixed with 10% formalin for 1 hour at room temperature. The oil globules accumulated by cells were stained using Oil Red O (3 mg/ml; Sigma-Aldrich, UK) for 1 hour at 37°C, after which they appeared red. The stained cells were observed under a phase contrast microscope, and the number of cells that had accumulated the oil globules was microscopically quantitated.
Adipogenic differentiation kit
Manufactured by Lonza
Sourced in Switzerland
The Adipogenic Differentiation Kit is a laboratory product designed to facilitate the differentiation of cells into adipocytes, or fat cells. It provides the necessary components and protocols for this process, allowing researchers to study adipogenesis and related cellular mechanisms.
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2 protocols using adipogenic differentiation kit
1
Adipogenic Differentiation of GMSCs
2
Multilineage Differentiation of Adherent Cells
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Cells were harvested from microcarriers after 7 passages in defined, xeno-free medium and re-plated on 6-well plates for directed differentiation into adipogenic, osteogenic, and chondrogenic lineages using the following kits: Adipogenic differentiation kit (Lonza, Cat. PT3102A/B), StemPro Osteogenic differentiation kit (Gibco, Cat. No. A10072-01) and StemPro Chondrogenic differentiation kit (Gibco, Cat. No. A10071-01). Stains used: Oil Red Staining Kit (Millipore), Alizarin Red Stain (Millipore), and 1% Alcian Blue Stain (Fisher Scientific). The protocols for differentiation and staining were performed following manufacturer’s recommendations.
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