The following chemical reagents were used in this study: Oxaliplatin (#HY‐17371; MedChemExpress, NJ, USA), 5‐Aza‐dC (#A3656; Sigma–Aldrich, MO, USA), Trichostatin A (#V900931; Sigma–Aldrich), chloroquine (#HY‐17589A; MedChemExpress), cycloheximide (#S7418; Selleck Chemicals, TX, USA), MG132 (#M7449; Sigma–Aldrich), RO‐3306 (#S7747; Selleck Chemicals), erastin (#S7242; Selleck Chemicals), RSL3 (#S8155; Selleck Chemicals), ferrostatin‐1 (#S7243; Selleck Chemicals), deferoxamine (#HY‐B1625; MedChemExpress), N‐acetyl‐cysteine (#HY‐B0215; MedChemExpress), Z‐VAD‐FMK (#V116; Sigma–Aldrich), necrostatin‐1 (#S8037; Selleck Chemicals), Rosiglitazone (#HY‐17386; MedChemExpress), and liproxstatin‐1 (#HY‐12726; MedChemExpress).
Deferoxamine
Manufactured by MedChemExpress
Sourced in United States, China
Deferoxamine is a chelating agent used in the laboratory to bind and remove iron from biological samples. It functions by forming stable complexes with ferric iron, facilitating its removal from cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Ferrostatin 1, by MedChemExpress (5 mentions)
Erastin, by MedChemExpress (5 mentions)
Z vad fmk, by MedChemExpress (3 mentions)
N acetyl l cysteine nac, by Beyotime (2 mentions)
Ab125066, by Abcam (2 mentions)
Necrosulfonamide, by MedChemExpress (2 mentions)
Anti slc7a11 nb300 318, by Novus Biologicals (2 mentions)
Anti ftl 10727 1 ap, by Proteintech (2 mentions)
Anti gstz1 gtx106109, by GeneTex (2 mentions)
Necrostatin 1, by Selleck Chemicals (1 mentions)
Mg132, by Merck Group (1 mentions)
Z vad fmk, by Merck Group (1 mentions)
Cycloheximide (chx), by Selleck Chemicals (1 mentions)
Trichostatin a, by Merck Group (1 mentions)
5 aza dc, by Merck Group (1 mentions)
N acetylcysteine, by MedChemExpress (1 mentions)
Chloroquine, by MedChemExpress (1 mentions)
Oxaliplatin, by MedChemExpress (1 mentions)
Ferrostatin 1, by Selleck Chemicals (1 mentions)
Ro 3306, by Selleck Chemicals (1 mentions)
6 protocols using deferoxamine
1
Pharmacological Modulation of Cell Death
2
Ferroptosis Pathway Modulator Protocol
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Erastin (HY-15763), Ferrostatin-1 (HY-100579), Deferoxamine (HY-B0988), and Necrosulfonamide (HY-100573) were purchased from MedChemExpress (MCE; Shanghai, China). Sorafenib (S7397), ZVAD-FMK (S7023), Bafilomycin A1 (S1413), and RSL3 (S8155) were obtained from Selleckchem (Houston, TX, USA). N-acetyl-L-cysteine (NAC, S0077) was from Beyotime (Shanghai, China). Brusatol (Bru, MB7292) was obtained from Meilunbio (Dalian, China). Tertiary butylhydroquinone (tBHQ, 112941) was obtained from Sigma (Shanghai, China). Antibodies raised against GPX4 (ab125066), NRF2 (ab62352), 4-HNE (ab46545), NQO1 (ab34173), and β-actin (ab6276) were obtained from Abcam (Cambridge, MA, USA), anti-SLC7A11 (NB300-318) was from Novusbio (Centennial, CO, USA), anti-FTL (10727-1-AP) was from Proteintech (Shanghai, China), and anti-GSTZ1 (GTX106109) was from GeneTex (San Antonio, CA, USA).
3
Investigating Ferroptosis Inhibitors in Cells
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DHA (purity ≥98% CAS: 71939‐50‐9) was bought from Yuanye Bio‐Technology Co., Ltd. (Shanghai, China). Puromycin was purchased from Sigma‐Aldrich Corporation (St. Louis, MO, USA). Hoechst 33342 fluorescence staining kit were purchased from Dojindo Laboratories Co., Ltd. (Kumamoto, Japan). Belnacasan, the protease inhibitor Z‐VAD‐FMK (benzyloxycarbonyl‐ValAla‐Asp (OMe) fluoromethylketone), ferrostatin‐1, erastin, deferoxamine and necrostatin‐1 were acquired from MedChemExpress (Monmouth Junction, NJ, USA). An apoptosis detection kit was got from BD Biosciences (San Jose, CA, USA). Foetal bovine serum (FBS) was obtained from Gibco (Thermo Fisher Scientific, Waltham, MA, USA).
4
Macrophage Ferroptosis Induction Assay
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Macrophages were implanted in a 96-well plate and cultured for 24 h at 37 °C. After macrophages had undergone different treatments at specific times, the supernatant was removed, and then the cells were washed with PBS three times. 100 μL 1640 medium containing 10 μL Cell Counting Kit-8 (CCK-8, Byotime, Beijing, China) was added into each well. The cells were incubated for 1 h at 37 °C until they turned orange and then measured at an absorbance of 450 nm. All drugs and corresponding treatment concentration: erastin (HY-15763), 10 μM; RSL3 (HY-100218A), 5 μM Ferrostatin-1 (HY-100579), 10 μM; deferoxamine (HY-B0988), 200 μM; necrostatin (HY-15760) 2 μM; ZVAD-FMK(HY-16658B), 5 μM were purchased from MedChemExpress (MCE). FeSO4 7H2O (F7002), 200 μg/mL; baicalein (465119), 20 μM were purchased from Sigma.
5
Ferroptosis Pathway Modulation Assay
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The corresponding reagents erastin (#HY-15763), sulfasalazine (#HY-14655), Z-VAD-FMK (#HY-16658B), E-necrosulfonamide (#HY-100573), ferrostatin-1 (#HY-100579), deferoxamine (#HY-D0903), and ciclopirox (#HY-B0450) were purchased from MedChemExpress. Liproxstatin-1 (#S7699) was purchased from Selleck. 3-Methyladenine (#189490) was purchased from Sigma Aldrich.
6
Ferroptosis Pathway Modulation Protocol
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Erastin (HY-15763), Ferrostatin-1 (HY-100579), Deferoxamine (HY-B0988) and Necrosulfonamide (HY-100573) were purchased from MedChemExpress (MCE; Shanghai, China). Sorafenib (S7397), ZVAD-FMK (S7023), Bafilomycin A1 (S1413) and RSL3 (S8155) were obtained from Selleckchem (Houston, TX, USA). N-acetyl-L-cysteine (NAC, S0077) was from Beyotime (Shanghai, China). Brusatol (Bru, MB7292) was obtained from Meilunbio (Dalian, China).
Tertiary butylhydroquinone (tBHQ, 112941) was obtained from Sigma (Shanghai, China). Antibodies raised against GPX4 (ab125066), NRF2 (ab62352), 4-HNE(ab46545), NQO1(ab34173) and β-actin (ab6276) were obtained from Abcam (Cambridge, MA, USA), anti-SLC7A11 (NB300-318) was from Novusbio (Centennial, CO, USA), anti-FTL (10727-1-AP) was from Proteintech (Shanghai, China), and anti-GSTZ1 (GTX106109) was from GeneTex (San Antonio, CA, USA).
Tertiary butylhydroquinone (tBHQ, 112941) was obtained from Sigma (Shanghai, China). Antibodies raised against GPX4 (ab125066), NRF2 (ab62352), 4-HNE(ab46545), NQO1(ab34173) and β-actin (ab6276) were obtained from Abcam (Cambridge, MA, USA), anti-SLC7A11 (NB300-318) was from Novusbio (Centennial, CO, USA), anti-FTL (10727-1-AP) was from Proteintech (Shanghai, China), and anti-GSTZ1 (GTX106109) was from GeneTex (San Antonio, CA, USA).
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