Hoechst 33342 h342

All chemicals were of analytical grade and, unless otherwise stated, obtained from Sigma-Aldrich (Steinheim, Germany), Merck (Darmstadt, Germany), and Roth (Karlsruhe, Germany), respectively. Propidium iodide (PI) was obtained from Sigma Aldrich (Steinheim, Germany), while Hoechst 33342 (H342) was purchased from Life Technologies (Darmstadt, Germany). Peanut agglutinin conjugated to fluorescein-isothiocyanate (PNA-FITC) and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1) were both from Enzo Life Sciences (Lörrach, Germany).

Antibodies recognizing SOX2, KLF4, NANOG, MRP2, BCRP, CUL3, p62, LC3, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and PSMB5 were from Cell Signaling Technology (Danvers, MA, USA). Antibodies against PSMB6 and PSMB7 were purchased from Enzo Life Sciences (Farmingdale, NJ, USA). NRF2, KEAP1, Ub, lamin B, and β-tubulin antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The luciferase reporter plasmid containing the ARE was a gift from Dr. Wakabayashi (University of Pittsburg, PA, USA). The lentiviral expression plasmids for human NRF2 or p62 short hairpin RNA (shRNA), Mission^TM Lentiviral Packaging Mix, hexadimethrine bromide, puromycin, 5-FU, doxorubicin, CHX, and MTT were from Sigma-Aldrich (Saint Louis, MO, USA). PI was purchased from Biolegend (San Diego, CA, USA). Hoechst 33342 (H342) and carboxy-H₂DCFDA were purchased from Life Technologies (Carlsbad, CA, USA). The SYBR premix ExTaq system was obtained from Takara (Otsu, Japan). Substrates for proteasome proteases (Suc-LLVY-AMC, Z-LLE-AMC, and Z-ARR-AMC) were from EMD Chemicals (Darmstadt, Germany).