All root canals were instrumented up to 60K file size (Sybron Endo). Irrigation was done using sodium hypochlorite (Parcan, Septodont healthcare) and normal saline to remove the remaining debris, dried with paper points, and filled with gutta-percha by lateral condensation and AH Plus sealer (DENTSPLY). The specimens were kept at 37°C and relative humidity for 7 days for setting of full cement. Then, the roots were embedded in acrylic resin. The post spaces were prepared using Peeso reamers (DENTSPLY) no 3 leaving 5 mm of gutta-percha in the apex.
Peeso reamers
Peeso reamers are a type of dental instrument used in endodontic procedures. They are designed to enlarge and shape the root canal before filling. Peeso reamers feature a series of flutes along the length of the instrument, which allows for efficient removal of dentin tissue during canal preparation.
Lab products found in correlation
5 protocols using peeso reamers
Standardized Root Canal Preparation
All root canals were instrumented up to 60K file size (Sybron Endo). Irrigation was done using sodium hypochlorite (Parcan, Septodont healthcare) and normal saline to remove the remaining debris, dried with paper points, and filled with gutta-percha by lateral condensation and AH Plus sealer (DENTSPLY). The specimens were kept at 37°C and relative humidity for 7 days for setting of full cement. Then, the roots were embedded in acrylic resin. The post spaces were prepared using Peeso reamers (DENTSPLY) no 3 leaving 5 mm of gutta-percha in the apex.
Standardized Fiber Post Placement Protocol
To simulate the periodontal ligament and alveolar bone, the root of each tooth was covered with a 0.2-mm layer of light body silicone impression material (Perfit; Hugedental) and then mounted in an auto-polymerizing acrylic resin (Shanghai Medical Instruments Co., Ltd, Shanghai, China) block up to 2mm below the CEJ before the fracture test.
Instrumentation of Immature Apices
Standardized Root Canal Preparation
Intact, single, straight, and conical-rooted human teeth (
n= 144) were selected for this study according to local university institutional review board (IRB) guidelines (IRB 1408889870). The teeth were saved for a maximum of 6 months after extraction at 4°C in 0.1% thymol solution until used. Samples were horizontally decoronated 0.5 mm apical to the facial/buccal cementoenamel junction using a water-cooled low-speed diamond saw (Buehler Ltd.; Lake Bluff, IL, USA Illinois, United States). Furthermore, the apical 3 mm of each root were removed, resulting in 8 ± 1 mm root sections. The internal diameter of the roots was standardized by mechanical preparation with Peeso reamers (Dentsply; Johnson City, Tennessee, United States) (size 1–5) to a final diameter of 1.5 mm. After the use of each size of Peeso reamer, root canals were irrigated with 2 mL of 1.5% sodium hypochlorite (NaOCl) for 1 minute using a 27-gauge needle. After instrumentation was completed, each canal received a final rinse with 5 mL of 1.5% NaOCl for 2 minutes, 5 mL of 17% ethylenediaminetetraacetic acid (EDTA) (Vista; Racine, Tennessee, United States) for 2 minutes and 5 mL of sterile water for 2 minutes.
Simulating Crown Fractures in Immature Teeth
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