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Ttl180 a

Manufactured by Thorlabs

The TTL180-A is a thorlabs-branded optical power meter. It measures the optical power of laser and other light sources. The device provides a digital readout of the measured power.

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3 protocols using ttl180 a

1

Surface Tension Measurement of Lipid-Buffer Interface

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A custom pendant droplet tensiometer was created to measure the surface tension of the TAG-buffer interface upon the addition of PLs. The droplet volume was controlled by a syringe pump (NanoJet, Chemyx) with a 1 mL TAG-filled syringe (1001 LT SYR, Hamilton) connected via a PEEK tubing to a 30-gauge blunt-tipped needle (930050–90BTE, Metcal) within a polystyrene cuvette (759076D, BrandTech). The droplet was imaged with CMOS camera (MU233-FL, AmScope), tube lens (TTL180-A, ThorLabs), and 4x air objective (PlanN, Olympus) after illumination with a diffused (DG10–1500, Thorlabs) desktop LED lamp (DLST01-S, Newhouse). Images were analyzed and the surface tension was extracted with FIJI and OpenDrop.31 (link),32 (link) FIJI was used to convert the
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2

Modular openSIM Illumination System

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Three high power PT54 LEDs (PT-54-TE, Luminus Devices; dominant wavelengths: red-amber λd  = 613 nm, green λd  = 525 nm, blue λd  = 460 nm, emitting area of 5.4 mm2) are used to generate the illumination light. The light emitted by the three LEDs is deflected by three mirrors (#43-875, Edmund optics), is collimated using 50 mm lenses (#66-018, Edmund optics), and combined into one optical path with dichroic mirrors (#69-898, #69-900, Edmund optics). A polarizer selects for the specific polarization reflected by the polarizing beam splitter (#49-002, Edmund optics). Then, the light reaches the spatial light modulator (QXGA-3DM-STR, ForthDimension Displays). A tube lens (TTL180-A, Thorlabs), compatible with the commercial microscope being used in this paper (IX71 and IX81, Olympus), is placed in such a way that the SLM is in the back focal place of that tube lens. Commercially available connectors (SM2Y1, LCP11, Thorlabs) were used to connect the openSIM to the illumination port of the microscope. The openSIM is designed to be easily adaptable to other commercially available microscopes (e.g. Olympus, Zeiss, Leica) by changing the tube lens and the illumination port adapter which are available in the Thorlabs catalog.
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3

Characterization of Metasurface Optical Response

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A 760.9 nm single frequency distributed feedback (DFB) laser (TOPTICA Eagleyard GmbH) is driven with a constant current source (Newport 505 Laser Diode Driver) and kept at a constant temperature (Newport 325 Thermoelectric Cooler Driver). The single mode fiber-coupled output is collimated with a reflective collimator (Thorlabs RC12APC-P01) and is incident on the fused silica face of the metasurface. The metasurface z-position is controlled using a closed-loop piezo-motor stage with nm resolution (Attocube ECSx3030). The transmitted light is captured using a horizontal microscope system comprising a high NA objective (Olympus 100x MPLAPON NA = 0.95), tube lens (Thorlabs TTL-180A) and CMOS camera (Thorlabs DCC1545M). The intensity image is captured over a range of longitudinal z-positions at steps of 50 nm, where z = 0 mm corresponds to the patterned surface of the metasurface. At each z-position, the system is allowed to stabilize for 10 s before multiple intensity images are captured at different exposure times ranging from 0.05 ms to 163 ms. These multiple exposure images are later weighted by their respective exposure times and stacked to remove saturated pixels and produce a composite image with a large intensity dynamic range.
Further experimental details are contained in Supplementary Information section 3.
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