For proliferation assay, transfected PC‐3, 22Rv1, and LNCaP cells were plated on 96‐well‐plates in densities of 6000, 7500, and 10 000 cells/well, respectively. Cell growth was monitored using IncuCyte live cell imaging system (Essen BioScience, Sartorius, Göttingen, Germany) for 6 days and the data was processed using IncuCyte 2021C software (Sartorius).
Incucyte 2021c software
Manufactured by Sartorius
Sourced in Germany
The IncuCyte 2021C software is a real-time, automated live-cell imaging and analysis system designed for use in life science research laboratories. It enables continuous monitoring and quantification of cell proliferation, migration, and other dynamic cellular processes over extended time periods.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using incucyte 2021c software
1
Cell Proliferation Assay for Prostate Cancer
2
Automated Wound Healing Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Approximately
25 000 cells
per well were seeded on Sartorius 96-well LockView or ImageLock microplates
(Sartorius AG) the night prior to wound formation. The cell monolayer
was scratched with the IncuCyte WoundMaker that simultaneously makes
equivalently sized scratch wounds in the monolayer in all wells, and
the medium was replaced with a compound-containing medium supplemented
with 1% fetal bovine serum (FBS) to induce cell migration and reduce
cell proliferation. Serial dilutions (1 μL) of the compounds
in DMSO were added to obtain the 1 nM to 10 μM final concentrations,
and the plate was mixed gently. The plates were placed in the IncuCyte
incubator and imaged in a bright field with a 10× objective every
3 h for 48 h. Analysis of the wound confluence and width was performed
with an automated script in IncuCyte 2021C software provided by Sartorius.
25 000 cells
per well were seeded on Sartorius 96-well LockView or ImageLock microplates
(Sartorius AG) the night prior to wound formation. The cell monolayer
was scratched with the IncuCyte WoundMaker that simultaneously makes
equivalently sized scratch wounds in the monolayer in all wells, and
the medium was replaced with a compound-containing medium supplemented
with 1% fetal bovine serum (FBS) to induce cell migration and reduce
cell proliferation. Serial dilutions (1 μL) of the compounds
in DMSO were added to obtain the 1 nM to 10 μM final concentrations,
and the plate was mixed gently. The plates were placed in the IncuCyte
incubator and imaged in a bright field with a 10× objective every
3 h for 48 h. Analysis of the wound confluence and width was performed
with an automated script in IncuCyte 2021C software provided by Sartorius.
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