Cytokines were measured in serum using the following multiplex assays: Bio-Plex Pro Mouse Cytokine 8-plex panel (#M60000007A) supplemented with IL-6 and IL-17A singleplex, Bio-Plex ProTM TGF-β 3-plex Assay (#171W4001M) (Bio-Rad), or ProcartaPlex Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse Panel (EPX170-26087-901) (Thermo Fisher Scientific). Antibody subclasses were measured using ProcartaPlex Mouse Antibody Isotyping 7-plex panel (EPX070-20815-901). In all cases the analyses were carried out following the manufacturers’ instructions, using a Luminex 200 reader and BioPlex Manager 6.0 software (BioRad). Analysis was performed on fluorescence index (FI) values minus background, while figures show concentrations calculated from standard curve. Analytes with more than 40% data points below limit of detection (Antweiler, 2015 (link)) were excluded from statistical evaluation.
Luminex 200 reader
Manufactured by Bio-Rad
Sourced in United States
The Luminex 200 reader is a multiplex assay system that uses color-coded magnetic beads to detect and quantify multiple analytes simultaneously in a single sample. The device utilizes flow cytometry technology to measure the fluorescent signals emitted by the beads, allowing for the rapid and efficient analysis of various biomolecules, such as proteins, cytokines, and nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using luminex 200 reader
1
Multiplex Cytokine and Antibody Profiling
2
Quantification of Cytokines and Chemokines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cytokines (IL-10, IFN-γ) and chemokines (IL-8, MCP-1) were quanti ed using custom-made ProcartaPlex® kits (Affymetrix Inc, City and/or State, U.S.A). The magnetic beads were quanti ed using a Luminex® 200 reader (Bio Rad, U.S.A) with the MasterPlex ® CT software (v1.0) with the following settings: i) sample size: 50 µL, ii) DD gate: 5,000-25,000, iii) timeout: 45 s, iv) bead event: 100. Data analysis was performed with the MasterPlex ® QT software (v2.0) with logistic 4-point weighting for standard curve tting. Endogenous concentrations of biomarkers in the sample were determined from this standard curve. The assay sensitivity of each biomarker of interest was: 2.71 pg/mL (IL-8), 2.49 pg/mL (IL-10), 11 pg/mL (IFN-γ), and 1.29 pg/mL (MCP-1).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!