The central area (~2 cm2) of placental tissue was obtained immediately after delivery from the preeclamptic and control groups. After washing of the maternal blood with physiological saline, tissues were immediately frozen in liquid nitrogen and stored. The rest of the fresh placental tissues were embedded in paraffin and cut to slices for immunohistochemistry. Total RNA was extracted from placenta using RNAiso Plus (Takara), and transcribed into cDNA by PrimeScript reagent Kit (Vazyme Biotech Co., Ltd). The protein was extracted using RIPA Lysis Buffer (Beyotime) and PMSF (Solarbio). BCA Protein Assay kit (CWBIO) was used to quantify the concentration of protein by Microplate Photometer (Thermo Fisher Scientific).
Primescript reagent kit
Manufactured by Vazyme
Sourced in China
The PrimeScript reagent Kit is a high-quality reverse transcription kit designed for cDNA synthesis. The kit includes necessary components for efficient and reliable conversion of RNA to cDNA, a crucial step in various molecular biology applications.
Automatically generated - may contain errors
Lab products found in correlation
Microplate photometer, by Thermo Fisher Scientific (1 mentions)
Rnaiso plus, by Takara Bio (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions)
Bca protein assay kit, by CWBIO (1 mentions)
Sybr green kit, by Thermo Fisher Scientific (1 mentions)
Abi stepone plus pcr instrument, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using primescript reagent kit
1
Placental Tissue Collection and Analysis
2
Cerebral Ischemia Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from cerebral ischemic penumbra tissues using TRIzol reagent (Invitrogen, Frederick, MD, USA) according to the manufacturer’s instructions. Then, RNA was reverse-transcribed into cDNA using a PrimeScript Reagent Kit (Vazyme, Nanjing, China). Quantitative real-time PCR was implemented on an ABI StepOne Plus PCR instrument (Applied Biosystems, CA, USA) with an SYBR green kit (Applied Biosystems). The relative gene expression levels were quantified and normalized to GAPDH. The primer sequences used were as follows:
Nos2: F: CAGCTGGGCTGTACAAACCTT and R: CATTGGAAGTGAAGCGTTTCG.
Il6: F:GCTGGTGACAACCACGGCCT and R: AGCCTCCGACTTGTGAAGTGGT;
Tnfa: F: CAAGGGACAAGGCTGCCCCG and R: GCAGGGGCTCTTGACGGCAG;
Ccl2:F: TTGAGGTGGTTGTGGAAAAGG and R: GTGCTGACCCCAAGAAGGAAT;
Il1b:F: AAGCCTCGTGCTGTCGGACC and R: TGAGGCCCAAGGCCACAGG;
Il10: F: GGTTGCCAAGCCTTATCGGA and R: ACCTGCTCCACTGCCTTGCT;
Gapdh: F: GCCAAGGCTGTGGGCAAGGT and R: TCTCCAGGCGGCACGTCAGA.
Nos2: F: CAGCTGGGCTGTACAAACCTT and R: CATTGGAAGTGAAGCGTTTCG.
Il6: F:GCTGGTGACAACCACGGCCT and R: AGCCTCCGACTTGTGAAGTGGT;
Tnfa: F: CAAGGGACAAGGCTGCCCCG and R: GCAGGGGCTCTTGACGGCAG;
Ccl2:F: TTGAGGTGGTTGTGGAAAAGG and R: GTGCTGACCCCAAGAAGGAAT;
Il1b:F: AAGCCTCGTGCTGTCGGACC and R: TGAGGCCCAAGGCCACAGG;
Il10: F: GGTTGCCAAGCCTTATCGGA and R: ACCTGCTCCACTGCCTTGCT;
Gapdh: F: GCCAAGGCTGTGGGCAAGGT and R: TCTCCAGGCGGCACGTCAGA.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!