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Auto western testing service

Manufactured by RayBiotech
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Sourced in United States

The Auto Western Testing Service provides automated western blot analysis. It streamlines the western blotting process, from sample preparation to data analysis.

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Lab products found in correlation

Protease and phosphatase inhibitor, by Merck Group (2 mentions) Ripa cell lysis buffer, by Merck Group (1 mentions)

2 protocols using auto western testing service

1

Automated Western Blot Protein Analysis

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RIPA cell lysis buffer supplemented with protease and phosphatase inhibitors (Sigma) was used for the extraction of total protein from either brain or vascular channel. The Auto Western Testing Service was provided by RayBiotech, Inc. (Peachtree Corners, GA USA). 0.2 mg/mL sample concentration was loaded into the automated capillary electrophoresis machine. Glial fibrillary acidic protein (GFAP) and Gluceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody provided by RayBiotech was used as the loading control.
Pediaditakis I., Kodella K.R., Manatakis D.V., Le C.Y., Barthakur S., Sorets A., Gravanis A., Ewart L., Rubin L.L., Manolakos E.S., Hinojosa C.D, & Karalis K. (2022). A microengineered Brain-Chip to model neuroinflammation in humans. iScience, 25(8), 104813.
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2

Detecting Phosphorylated α-Synuclein in Cells

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RIPA cell lysis buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 5 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate, and 1% SDS) supplemented with protease and phosphatase inhibitors (Sigma) was used for the extraction of total protein from either brain or vascular channel. The Auto Western Testing Service was provided by RayBiotech, Inc. (Peachtree Corners, GA USA). 0.1 mg/mL sample concentration was loaded into the automated capillary electrophoresis machine. Phosphorylated αSyn at Ser129 was detected with a pSer129-α-syn antibody (1:100;Cell Signaling, 23706). Gluceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody provided by RayBiotech (service library antibody) was used as the loading control. The uncropped WB images are provided in the source data file.
Pediaditakis I., Kodella K.R., Manatakis D.V., Le C.Y., Hinojosa C.D., Tien-Street W., Manolakos E.S., Vekrellis K., Hamilton G.A., Ewart L., Rubin L.L, & Karalis K. (2021). Modeling alpha-synuclein pathology in a human brain-chip to assess blood-brain barrier disruption. Nature Communications, 12, 5907.
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