Nova1

Manufactured by Abcam

Sourced in United Kingdom

NOVA1 is a protein that functions as a regulator of gene expression. It is involved in the post-transcriptional regulation of target mRNAs. NOVA1 contains three K-homology (KH) domains which are important for its RNA-binding activity.

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Lab products found in correlation

Ventana bench mark xt autostainer, by Roche (2 mentions) Bond 3 autostainer, by Leica (1 mentions) Alpha smooth muscle actin, by Agilent Technologies (1 mentions) Clone 1a4, by Agilent Technologies (1 mentions) Jnk1 2, by BD (1 mentions) Pser63 c jun, by Cell Signaling Technology (1 mentions) β tubulin, by BioLegend (1 mentions) Flag m2, by Merck Group (1 mentions) C jun, by Cell Signaling Technology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-NOVA1 (4 citations)

3 protocols using nova1

Comprehensive Immunohistochemistry Profiling

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Immunohistochemistry was performed using a Ventana Bench Mark XT Autostainer (Ventana Medical Systems, Tucson, AZ, USA) and a LEICA BOND-III Autostainer (Leica Biosystems, Newcastle Upon Tyne, UK). Tested primary antibodies were as follows: NOVA1 (dilution 1:500; Abcam, Cambridge, UK), CD68 (dilution 1:150; DAKO), CD20 (dilution 1:1600; clone L26; DAKO), CD3 (dilution 1:200; LabVision, Fremont, CA, USA),
CD4 (dilution 1:200; Cell Marque, Rocklin, CA, USA), FOXP3 (dilution 1:100; Aviva Systems Biology, CA, USA), myeloperoxidase (dilution 1: 2000, DAKO), CD34 (dilution 1:50; clone QBEnd 10; DAKO), S100 (dilution 1:2000; DAKO), and alpha smooth muscle actin (dilution 1:500; clone 1A4; DAKO). Double staining with NOVA1/CD20, NOVA1/CD3, NOVA1/CD21, and NOVA1/CD68 was performed using palatine tonsil tissues for cellular localization of NOVA1 expression. Cell typing of immune cells and stromal spindle cells was also confirmed histomorphologically with H&E and immunohistochemical stains as follows: macrophages/monocytes/dendritic cells (CD68, S100, myeloperoxidase), B-lymphocytes (CD20), T-lymphocytes (CD3), regulatory T cells (FOXP3), neutrophils (myeloperoxidase), stromal spindle cells (Schwann cells, fibroblasts, support cells, and endothelial cells; S100, smooth muscle actin, and CD34).

Yoon S.O., Kim E.K., Lee M., Jung W.Y., Lee H., Kang Y., Jang Y.J., Hong S.W., Choi S.H, & Yang W.I. (2015). NOVA1 inhibition by miR-146b-5p in the remnant tissue microenvironment defines occult residual disease after gastric cancer removal. Oncotarget, 7(3), 2475-2495.

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Western Blot Analysis of cJUN Signaling

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Tissue extracts were prepared using Triton lysis buffer (20 mM Tris-pH 7.4, 1% Triton-X100, 10% glycerol, 137 mM NaCl, 2 mM EDTA, 25 mM β-glycerophosphate, 1 mM sodium orthovanadate, 1 mM PMSF and 10 µg/mL leupeptin plus aprotinin). Extracts (30–50 µg of protein) were examined by immunoblot analysis by probing with antibodies to cJUN (Cell Signaling Technology Cat# 9165L, RRID:AB_2129578), pSer⁶³-cJUN (Cell Signaling Technology Cat# 9261S, RRID:AB_2130162), Flag M2 (Sigma-Aldrich Cat# F1804, RRID:AB_262044), GAPDH (Santa Cruz Biotechnology Cat# sc-365062, RRID:AB_10847862), JNK1/2 (BD Biosciences Cat# 554285, RRID:AB_395344), pThr¹⁸⁰-Pro-pTyr¹⁸²-JNK (pJNK) (Cell Signaling Technology Cat# 4668P, RRID:AB_10831195), NOVA1 (Abcam Cat# ab97368, RRID:AB_10680798), NOVA2 (Sigma-Aldrich Cat# AV40400, RRID:AB_1854572), and βTubulin (BioLegend Cat# 903401, RRID:AB_2565030). Immunocomplexes were detected by fluorescence using anti-mouse and anti-rabbit secondary IRDye antibodies (Li-Cor) and quantitated using the Li-Cor Imaging system

Vernia S., Edwards Y.J., Han M.S., Cavanagh-Kyros J., Barrett T., Kim J.K, & Davis R.J. (2016). An alternative splicing program promotes adipose tissue thermogenesis. eLife, 5, e17672.

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Tissue Microarray Analysis of NOVA1 Expression

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Sections of FFPE tissues were prepared and stained with hematoxylin and eosin. Under the microscope, the representative area was confirmed and selected for a 3-mm-core sized tissue microarray (TMA). One or two different representative areas per case were selected and 29 cores were embedded in a TMA block.
Immunohistochemistry of NOVA1 (1:500, Abcam, Cambridge, UK) was performed on 4-μm tissue sections using the Ventana BenchMark XT Autostainer (Ventana Medical Systems, Tucson, AZ, USA). Immunohistochemistry with other primary antibodies was performed for diagnostic purposes and the tested antibodies are listed in Appendix 2.

Kim E.K., Yoon S.O., Kim S.H., Yang W.I., Cho Y.A, & Kim S.J. (2016). Upregulated Neuro-oncological Ventral Antigen 1 (NOVA1) Expression Is Specific to Mature and Immature T- and NK-Cell Lymphomas. Journal of Pathology and Translational Medicine, 50(2), 104-112.

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