Antibodies against tgr5

Manufactured by Abcam

Sourced in United Kingdom

Antibodies against TGR5, also known as Membrane-type Bile Acid Receptor or G-Protein Coupled Bile Acid Receptor 1, are laboratory reagents used to detect and study this cell surface receptor. TGR5 is involved in the regulation of bile acid homeostasis and energy metabolism.

Automatically generated - may contain errors

Lab products found in correlation

Stattic, by MedChemExpress (1 mentions) Int 777, by MedChemExpress (1 mentions) Antibodies against phospho p38, by Cell Signaling Technology (1 mentions) Phospho jnk, by Cell Signaling Technology (1 mentions) Gw4064, by MedChemExpress (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) Phospho p65, by Cell Signaling Technology (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) β actin, by ABclonal (1 mentions) Betulinic acid, by Bio-Techne (1 mentions) Neun antibody, by Merck Group (1 mentions) Real time pcr rt pcr primers, by Qiagen (1 mentions) Antibodies against, by Fujifilm (1 mentions) Glast pe, by Miltenyi Biotec (1 mentions)

2 protocols using antibodies against tgr5

LPS-Induced Inflammation Modulation

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LPS (Escherichia coli strain O111:B4) was obtained from Sigma (L2630; Sigma, St. Louis, MO, USA). PZH powder was obtained from Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd (Zhangzhou, China). The powder was dissolved in phosphate buffered solution (PBS) and ultrasonicated at 70 Hz for 40 min. Then, the supernatant was collected for in vitro assays, and the suspension was used for the in vivo assays. Stattic (Cat# HY-13818), INT-777 (Cat# HY-15677), INT-767 (Cat# HY-12434) and GW4064 (Cat# HY-50108) were purchased from MedChemExpress (Monmouth Junction, NJ, USA) and dissolved in dimethyl sulfoxide (DMSO). Cholestyramine resin was purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China). Antibodies against phospho-p38 (Cat# 4511s), p38 (Cat# 9218s), phospho-JNK (Cat# 4668s), JNK (Cat# 9252s), phospho-ERK1/2 (Cat# 4377s), ERK1/2 (Cat# 9102s), phospho-p65 (Cat# 3033s), p65 (Cat# 8242s), A20 (Cat# 5630s), and STAT3 (Cat# 9139s, CST) were purchased from Cell Signalling Technology (Boston, MA, USA). Antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cat# AC002), β-actin (Cat# AC002) and phospho-STAT3 (Cat# AC004) were obtained from ABclonal Technology (Wuhan, China). Antibodies against TGR5 (Cat# ab72608) were obtained from Abcam (Cambridge, UK).

Li B., Zhang Y., Liu X., Zhang Z., Zhuang S., Zhong X., Chen W., Hong Y., Mo P., Lin S., Wang S, & Yu C. (2023). Traditional Chinese medicine Pien-Tze-Huang ameliorates LPS-induced sepsis through bile acid-mediated activation of TGR5-STAT3-A20 signalling. Journal of Pharmaceutical Analysis, 14(4), 100915.

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Immunohistochemical Analysis of Microglia

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Antibodies against ionized calcium-binding adapter molecule 1 (IBA1) were purchased from Wako Chemicals USA (Richmond, VA). Antibodies against TGR5 were purchased from Abcam (Cambridge, MA). Antibodies against CD11b, glutamate aspartate transporter (GLAST), CD11b-phycoerythrin (PE), CD90-PE, and GLAST-PE were purchased from Miltenyi Biotec (San Diego, CA). NeuN antibodies were ordered from Millipore (Billerica, MA). All real-time PCR (RT-PCR) primers were purchased from SABiosciences (Frederick, MD). Betulinic acid was purchased from Tocris Bioscience (Minneapolis, MN). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted, and were of the highest grade available.

McMillin M., Frampton G., Tobin R., Dusio G., Smith J., Shin H., Newell-Rogers M.K., Grant S, & DeMorrow S. (2015). TGR5 signaling reduces neuroinflammation during hepatic encephalopathy. Journal of neurochemistry, 135(3), 565-576.

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