Donkey antigoat igg irdye 680

Reagents were obtained from the following sources: Cholesteryl BODIPY FLC12 (cholesteryl 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacence-3-dodecanoate) was obtained from Molecular Probes (Life Technologies, Grand Island, NY). Bicinchoninic acid assay protein kit was from Pierce Biotechnology, Inc. (Rockford, IL); organic solvents were from J. T. Baker (Phillipsburg, NJ); TRIzol reagent and SuperScript II were from Invitrogen (Carlsbad, CA); RNeasy kit was from QIAGEN (Valencia, CA); SyBr green Taqman PCR kit was from Applied Biosystems (Foster City, CA); Odyssey blocking buffer, goat antimouse IgG-IRDye 800, donkey antigoat IgG-IRDye 680, and goat antirabbit IgG-IRDye 680 were from Li-Cor Biosciences (Lincoln, NE); block lipid transport-1 (BLT-1), sulfo-N-succinimidyl oleate (SSO), isobutyl-methylxanthine, dexamethasone, and insulin were from Sigma-Aldrich (St. Louis, MO). QBT Fatty Acid Uptake Assay Kit was from Molecular Devices Corporation, Sunnyvale, CA, USA; siRNAs for mouse SR-B1 and CD36 were purchased from Thermo Fisher Scientific (Waltham, MA).

Reagents were obtained from the following sources: Cholesterol LiquiColor Test (Enzymatic) from Stanbio (Boerne, TX, USA); bicinchoninic acid assay protein kit from Pierce Biotechnology, Inc. (Rockford, IL, USA); organic solvents were from J. T. Baker (Phillipsburg, NJ, USA); TRIzol reagent and SuperScript II from Invitrogen (Carlsbad, CA, USA); RNeasy kit from QIAGEN (Valencia, CA, USA); SyBr green Taqman PCR kit from Applied Biosystems (Foster City, CA, USA); Odyssey blocking buffer, goat anti-mouse IgG-IRDye 800, donkey anti-goat IgG-IRDye 680, and goat anti-rabbit IgG-IRDye 680 from Li-Cor Biosciences (Lincoln, NE, USA); corticosterone ELISA kit from Cayman Chemical (Ann Arbor, Michigan, USA); progesterone and testosterone ELISA kits from ALPCO (Salem, NH, USA); pregnant mare’s serum gonadotropin (PMSG)/equine chorionic gonadotropin (eCG), and Bt₂cAMP from Sigma (St. Louis, MO, USA); ³H₂O and ³H-palmitic acid were from PerkinElmer (Waltham, MA, USA). Cortrosyn was from Amphastar Pharmaceuticals Inc. (Rancho Cucamonga, CA, USA).