Cells were transfected in culture with siRNA (35 nM, Dharmacon) or plasmid using RNAiMax (Life Technologies) or TransIT (Mirus Bio), respectively. Subsequently, cells were seeded on glass cover slips and fixed with 4% formaldehyde/PBS for 10 min at room temperature. After washing with PBS, cells were permeabilized with 0.1% Triton-X100/PBS at room temperature for 5 min. Following another wash step, actin filaments were stained with California Red-conjugated phalloidin (Biomol) for 20 min at room temperature. After washing with PBS and rinsing with H2O, the cells were covered with mounting medium (Vectashield). Microscopy was performed on a Leica AF6000 3D Deconvolution microscope system.
Af6000 3d deconvolution microscope system
Manufactured by Leica
The AF6000 3D Deconvolution microscope system is a lab equipment product from Leica. It is designed for advanced 3D imaging and analysis, utilizing deconvolution technology to enhance image quality and resolution.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using af6000 3d deconvolution microscope system
1
Visualizing Cellular Actin Filaments
2
Immunofluorescence Analysis of Actin Cytoskeleton
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