Mix-stage C. elegans animals were harvested with M9, bleached with 12% NaOCl and 1.5 M NaOH, and centrifuged at 500 × g to remove bacteria and debris. Embryos were washed 3 times with ddH2O, mounted on poly-L-lysine coated slides, and frozen at −80° for 10 minutes. They were fixed with Methanol for 15 minutes on ice and washed with PBS before being stained with anti-CNT-1 antibody at 1:500 dilution in PBS for 1 hour. After the embryos were washed three times with PBS, they were stained with Alexa Fluor 488–conjugated anti-rat IgG antibody (Molecular Probes #A-11006; validation is provided on the manufacturer’s website) at 1:1,000 dilution in PBS for 1 hour. They were then washed three times with PBS and visualized using an Axioplan 2 Nomarski Microscope (Carl Zeiss MicroImaging Inc.).
Axioplan 2 nomarski microscope
Manufactured by Zeiss
The Axioplan 2 Nomarski Microscope is a laboratory equipment designed for high-resolution optical microscopy. It utilizes the Nomarski Differential Interference Contrast (DIC) technique to enhance the visualization of transparent or weakly stained specimens. The core function of this microscope is to provide detailed and high-contrast images of the sample under investigation.
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Lab products found in correlation
2 protocols using axioplan 2 nomarski microscope
1
Immunofluorescence Imaging of CNT-1 in C. elegans
2
Immunofluorescence Imaging of CNT-1 in C. elegans
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Mix-stage C. elegans animals were harvested with M9, bleached with 12% NaOCl and 1.5 M NaOH, and centrifuged at 500 × g to remove bacteria and debris. Embryos were washed 3 times with ddH2O, mounted on poly-L-lysine coated slides, and frozen at −80° for 10 minutes. They were fixed with Methanol for 15 minutes on ice and washed with PBS before being stained with anti-CNT-1 antibody at 1:500 dilution in PBS for 1 hour. After the embryos were washed three times with PBS, they were stained with Alexa Fluor 488–conjugated anti-rat IgG antibody (Molecular Probes #A-11006; validation is provided on the manufacturer’s website) at 1:1,000 dilution in PBS for 1 hour. They were then washed three times with PBS and visualized using an Axioplan 2 Nomarski Microscope (Carl Zeiss MicroImaging Inc.).
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