C57bl 6 tg cag flpe 36ito itorbrc

Mice were housed in the SPF animal facilities of the Central Animal Facility (PDC) of the Leiden University Medical Center (LUMC). The health status of the animals was monitored over time. Animals tested negative for all agents listed in the FELASA (Federation of European Laboratory Animal Science Associations) guidelines for SPF mouse colonies. All mouse studies were approved by the animal ethics committee of the LUMC. Experiments were performed in accordance with the Dutch Act on Animal Experimentation and EU Directive 2010/63/EU (‘On the protection of animals used for scientific purposes’). C57BL/6J mice were purchased from Charles River laboratories. FcγR^−/− mice were generated in the transgenic mouse facility of the LUMC (i.e., FcγRI^−/− [28 (link)], FcγRI/II/III/IV^−/− [29 (link)]. The generation of FcγRIV^−/− and FcγRIII/IV^−/− mouse strains is described in the Supplementary Data (Supplementary Figures 1 and 2). The EIIaCre deleter strain (>20 generations backcrossed on a C57BL/6J background), was a kind gift of Dr. Heiner Westphal. The Flp deleter strain C57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc (>20 generations backcrossed on a C57BL/6J background) was purchased from Jackson Laboratories (Bar Harbor, Me). Mice were routinely checked for their genotype by PCR analysis.