miR-29a mimics (miR-29a) and scrambled miRNA (Scrambled), siRNA for DNMT3A (si-DNMT3A), siRNA for DNMT3B (si-DNMT3B) and negative control (siRNA-NC) were purchased from Shanghai GenePharma Co., Ltd., and the sequences are presented in Table I . HeLa and C-33A cells were seeded into 6-well plates at a density of 2×105 cells/well and cultured at 37°C for 24 h, prior to transfection using the Lipofectamine® 2000 kit (cat. no. 11668027; Invitrogen, Thermo Fisher Scientific, Inc.) at 37°C for 48 h, according to the manufacturer's protocol. The final concentrations of miR-29a mimics and siRNAs were 50 nM and 80 nM, respectively. Cells were harvested 24 h post-transfection.
Mir 29a
Manufactured by GenePharma
The MiR-29a is a lab equipment product developed by GenePharma. It functions as a real-time PCR (polymerase chain reaction) detection system for the analysis of microRNA-29a expression levels.
Automatically generated - may contain errors
2 protocols using mir 29a
1
Transfection of miR-29a and DNMT3A/B in Cervical Cancer Cells
2
Transfecting miR-29a and anti-miR-29 in CFs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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miR-29a and its inhibitor anti-miR-29 (AMO-29) were synthesized by Genepharma (Shanghai). The sequence of miR-29a is: 5′-UAGCACCAUCUGAAAUCGGUUA-3′. The sequence ofAMO-29a is 5′-UAACCGAUUUCAGAUGGUGCUA-3′. miRNAs were transfected to cultured CFs using X-tremeGENE siRNA Transfection Reagent (Roche, Germany).
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