Pre cleaned glass slides

Sphingosine 5/6-fluorescein (SF) and sphingosine-1-phosphate 5/6-fluorescein (S1PF) were procured from Echelon Biosciences Inc. (Salt Lake City, UT). Monosodium phosphate, 1-propanol, isopropanol, methanol, and pre-cleaned glass slides (50 mm × 45 mm × 1.5 mm) were acquired from Fisher Scientific (Pittsburgh, PA). Sodium chloride, sodium azide, potassium chloride, paraformaldehyde, magnesium chloride, calcium chloride, piperazine-1-ethanesulfonic acid (HEPES), formic acid, and glucose were purchased from Sigma-Aldrich Inc. (St Louis, MO). Ethanol was procured from Decon Labs (King of Prussia, PA). Roswell Park Memorial Institute Media (RPMI) with l-glutamine, fetal bovine serum (FBS), Dulbecco’s phosphate buffered saline, and penicillin/streptomycin were purchased from Invitrogen (Grand Island, NY). EPON resin 1002F (phenol, 4,4′-(1-methylethylidene)bis-, polymer with 2,2′-[(1-methylethylidene)-bis(4,1-phenyleneoxymethylene)]bis-[oxirane]) was acquired from Miller–Stephenson (Sylmar, CA, USA). SU-8 developer (1-methoxy-2-propyl acetate) was purchased from MicroChem (Newton, MA, USA). Poly(dimethylsiloxane) (PDMS) (Sylgard 184 Silicone Elastomer) was procured from Dow Corning (Midland, MI). Acetonitrile was purchased from VWR (Radnor, PA).

Sphingosine 5/6-fluorescein (SF) and sphingosine-1-phosphate 5/6-fluorescein (S1PF) were purchased from Echelon Biosciences Inc. (Salt Lake City, UT). Sodium chloride, potassium chloride, magnesium chloride, calcium chloride, piperazine-1-ethanesulfonic acid (HEPES), and glucose were acquired from Sigma-Aldrich Inc. (St. Louis, MO). Monosodium phosphate, 1-propanol, mEthanol, isopropanol, and pre-cleaned glass slides (50 mm × 45 mm × 1.5 mm) were procured from Fisher Scientific (Pittsburgh, PA). Ethanol was acquired from Decon Labs (King of Prussia, PA). Roswell Park Memorial Institute Media (RPMI) was purchased from Cellgro (Manassas, VA) and Adoptive Immunotherapy Media (AIM-V®) was acquired from Gibco (Grand Island, NY). Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Lawrenceville, GA), human AB serum (HS) from Gemini BioProducts, and penicillin/streptomycin from Gibco (Grand Island, NY). EPON resin 1002F (phenol, 4,4'-(1-methylethylidene) bis-, polymer with 2,2'-[(1-methylethylidene) bis (4,1-phenyleneoxymethylene)]bis-[oxirane]) was procured from Miller-Stephenson (Sylmar, CA, USA). SU-8 developer (1-methoxy-2-propyl acetate) was acquired from MicroChem (Newton, MA, USA). Poly(dimethylsiloxane) (PDMS) (Slygard 184 Silicone Elastomer) was purchased from Dow Corning (Midland, MI).