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Dharmafect 2 transfection reagent

Manufactured by GE Healthcare
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Sourced in United Kingdom

DharmaFECT-2 is a lipid-based transfection reagent designed for efficient delivery of small interfering RNA (siRNA) and other nucleic acids into a variety of cell types. The reagent facilitates the uptake of nucleic acid molecules into the target cells, enabling gene silencing or other gene expression studies.

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Lab products found in correlation

On targetplus smartpool sirna, by GE Healthcare (2 mentions) Control sirna, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

DharmaFECT transfection reagent (29 citations) DharmaFECT (22 citations) Dharmafect-2 (9 citations) Dharmafect reagent (7 citations) Dharmafect-2 reagent (3 citations)

3 protocols using dharmafect 2 transfection reagent

1

Transfection and Viability Assays

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24 hours before transfection, cells were seeded in media containing 10% FBS without antibiotics. Cells were then transfected with control siRNA (Cell Signaling), PRMT-1 siRNA (Dharmacon), or p120-catenin siRNA (Santa Cruz Biotechnology) using Dharmafect-2 transfection reagent, (GE Life Sciences) per manufacturer’s protocol. Cell lysates were collected for immunoblotting after 24 and 48 hours. For MTT cell viability assays, the transfection media was removed after 24 hours followed by addition of drug media.
Iderzorig T., Kellen J., Osude C., Singh S., Woodman J.A., Garcia C, & Puri N. (2018). Comparison of Epithelial Mesenchymal Transition Mediated Tyrosine Kinase Inhibitor Resistance in Non-Small Cell Lung Cancer Cell Lines with Wild Type EGFR and Mutant Type EGFR. Biochemical and biophysical research communications, 496(2), 770-777.
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2

siRNA Knockdown of Smad2 and Smad3

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For siRNA knockdown experiments, 1.0 × 105 cells/well were seeded into 6-well plates and were transfected with 100nM of ON-TARGETplus SMARTpool siRNAs (GE Healthcare, Little Chalfont, UK) specific for Smad2, Smad3, or a non-targeting control using 4 μL DharmaFECT 2 transfection reagent (GE Healthcare) per transfection according to the manufacturer's instructions. Cells were incubated overnight, and then treated with TGF-β1 for 3 d before use.
David J.M., Dominguez C., McCampbell K.K., Gulley J.L., Schlom J, & Palena C. (2017). A novel bifunctional anti-PD-L1/TGF-β Trap fusion protein (M7824) efficiently reverts mesenchymalization of human lung cancer cells. Oncoimmunology, 6(10), e1349589.
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3

siRNA Analysis of MR and AhR

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Small interfering RNA (siRNA) analysis was carried out as previously described with slight modification. 5, 6 MR and AhR siRNA were the SMARTpool:ON-TARGETplus siRNA from GE Healthcare (Little Chalfont, United Kingdom). The control siRNA was the ON-TARGETplus Nontargeting control from GE Healthcare. Monocytes were transfected on day 0 with 50 nmol/L siRNA by using the DharmaFECT 2 Transfection Reagent (GE Healthcare). Inhibition was assessed at day 6.
Salazar F., Hall L., Negm O.H., Awuah D., Tighe P.J., Shakib F, & Ghaemmaghami A.M. (2016). The mannose receptor negatively modulates the Toll-like receptor 4-aryl hydrocarbon receptor-indoleamine 2,3-dioxygenase axis in dendritic cells affecting T helper cell polarization. The Journal of allergy and clinical immunology, 137(6).
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