Focal demyelinated lesions were induced by injecting 1 μl of 1.0% LPC (Sigma-Aldrich, MA, USA) in PBS into the spinal cord ventral funiculus of 11-week-old mice as previously described (Psachoulia et al., 2016 (link)). For the treatment group, 1 μl of 1 mg/ml recombinant human HMGB1 protein (Abcam, Cambridge, UK) in water was co-injected along with 1.0% LPC into the ventral spinal cord. For the control group, 1 μl of 1 mg/ml recombinant human HMGB1 protein (Abcam, Cambridge, UK) in water was co-injected with the vehicle, sterile phosphate buffered saline (PBS).
Rouillard M.E., Hu J., Sutter P.A., Kim H.W., Huang J.K, & Crocker S.J. (2022). The Cellular Senescence Factor Extracellular HMGB1 Directly Inhibits Oligodendrocyte Progenitor Cell Differentiation and Impairs CNS Remyelination. Frontiers in Cellular Neuroscience, 16, 833186.
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