Anti mouse igg dylight 800

Manufactured by Thermo Fisher Scientific

The Anti-mouse IgG–DyLight 800 is a fluorescent-labeled secondary antibody designed for detection of mouse immunoglobulin G (IgG) in various immunoassay applications. The antibody is conjugated with the DyLight 800 fluorescent dye, which has an excitation/emission maxima of 777/794 nm.

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Lab products found in correlation

Anti rabbit igg dylight 680, by Thermo Fisher Scientific (2 mentions) Anti rabbit igg dylight 488, by Thermo Fisher Scientific (2 mentions) Anti mouse igg h l alexafluor488, by Thermo Fisher Scientific (1 mentions) Anti flag m2 magnetic bead, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Anti ubiquitin p4d1, by Santa Cruz Biotechnology (1 mentions) Anti actin, by Santa Cruz Biotechnology (1 mentions) Anti flag m2, by Merck Group (1 mentions) Anti ate1, by Santa Cruz Biotechnology (1 mentions) Anti mouse igg dylight680, by Thermo Fisher Scientific (1 mentions) Mg132, by Cayman Chemical (1 mentions) Odyssey blocking buffer, by LI COR (1 mentions) Minitab, by Roche (1 mentions) Odyssey protein imaging system, by LI COR (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Hrp linked antibody, by Cell Signaling Technology (1 mentions) Itgb2, by Cell Signaling Technology (1 mentions) Glut4, by Cell Signaling Technology (1 mentions) Pdk4 12949 1 ap, by Proteintech (1 mentions) Ldhb 14824 1 ap, by Proteintech (1 mentions)

Spelling variants of this product

DyLight 800 (57 citations) Goat anti-mouse IgG DyLight 800 conjugate (6 citations) Anti-mouse DyLight 800 (5 citations) Goat anti-mouse IgG Dylight 800 (4 citations) Mouse anti-IgG (2 citations)

3 protocols using anti mouse igg dylight 800

Proteomic Analysis of TDP-43 Regulation

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Cycloheximide was obtained from Sigma-Aldrich. MG132 was obtained from Cayman Chemical. Chloroquine was obtained from Invitrogen/Thermo Scientific. Anti-C-terminal TDP43 (catalog number 12892-1-AP) and anti-phospho-TDP43 (catalog number 66318-1-Ig) were obtained from Proteintech. Anti-FLAG M2 (catalog number F1804) and Anti-FLAG M2 magnetic beads (catalog number M8823) were obtained from Sigma-Aldrich. Anti-ATE1 (catalog number sc-398805), antiactin (catalog number sc-47778), and anti-ubiquitin P4D1 (catalog number sc-8017) were obtained from Santa Cruz Biotechnology. Detection was carried out by using the following goat secondary antibodies from Thermo Scientific: anti-mouse IgG(H+L)–Alexa Fluor 488 (catalog number A-11001), anti-rabbit IgG(H+L)–Alexa Fluor 546 (catalog number A11010), anti-mouse IgG–DyLight 680 (catalog number PI35519), anti-rabbit IgG–DyLight 680 (catalog number SA5-10176), anti-mouse IgG–DyLight 800 (catalog number PI35569), and anti-rabbit IgG–DyLight 680 (catalog number SA5-10036).

Kasu Y.A., Alemu S., Lamari A., Loew N, & Brower C.S. (2018). The N Termini of TAR DNA-Binding Protein 43 (TDP43) C-Terminal Fragments Influence Degradation, Aggregation Propensity, and Morphology. Molecular and Cellular Biology, 38(19), e00243-18.

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Western Blot Analysis of MAPK Signaling

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Whole-cell lysates were extracted using RIPA lysis buffer and a mixture of protease and phosphatase inhibitors (Minitab, Roche, Basel, Switzerland). Lysates were sonicated on ice, debris removed by centrifugation, and supernatants stored at −80 °C. Sodium dodecyl sulfate-polyacrylamide 12% gels were used to separate proteins, and proteins were transferred to nitrocellulose membranes. Membranes were blocked with Odyssey blocking buffer (Li-Cor, Lincoln, NE) in a 1:1 mixture with PBS 1% Tween-20 (PBST). Primary antibodies (phospho-MAPK, and total MAPK, Cell Signaling, Danvers, MA) were incubated overnight in 1:1 blocking buffer to PBST. Primary antibodies were detected using DyLight conjugated secondary antibodies (anti-rabbit IgG DyLight 488 (#35553), and anti-mouse IgG DyLight 800 (#35521) from Thermo Fisher (Waltham, MA)). Protein bands were detected using Li-Cor odyssey protein imaging system.

Kumar D., Yalamanchali S., New J., Parsel S., New N., Holcomb A., Gunewardena S., Tawfik O., Lominska C., Kimler B.F., Anant S., Kakarala K., Tsue T.T., Shnayder Y., Sykes K., Padhye S, & Thomas S.M. (2018). Development and characterization of an in vitro model for radiation-induced fibrosis. Radiation research, 189(3), 326-336.

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Quantitative Analysis of Metabolic Regulators

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ITGB2 (#73663), GluT4 (#2213), Akt (#4691), Phospho-Akt (#4060), PI3Kinase (#4292), Anti-rabbit IgG, HRP-linked Antibody (#7074), Anti-mouse IgG and HRP-linked Antibody (#7076) were obtained from Cell Signaling Technology. ITGB2 (ab53009), Phospho-PI3Kinase (ab182651) and a-SMA (ab5694) were obtained from the Abcam company. ITGB2 (10554-1-AP), HIF1a (66730-1-Ig), MCT1 (20139-1-AP), MCT4 (22787-1-AP), LDHB (14824-1-AP) and PDK4 (12949-1-AP) were obtained from the Proteintech company. ITGB2 (AF1730) was purchased from R&D. Secondary anti-rabbit IgG Dylight 680 (#35568), anti-rabbit IgG Dylight 488 (#35553) and anti-mouse IgG Dylight 800 (#35521) were obtained from ThermoFisher.
The primer sequences used in this study were obtained from commercial sources and are displayed in Table S1.

Zhang X., Dong Y., Zhao M., Ding L., Yang X., Jing Y., Song Y., Chen S., Hu Q, & Ni Y. (2020). ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system. Theranostics, 10(26), 12044-12059.

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