Uv vis spectrophotometer 118

Method outlined by Rosset et al. (1982) was followed to assess the total phenolics content of mushroom flours. Samples (100 mg) were extracted with methanol (50%; 5 ml) in water bath (95 ± 1°C; 10 min) and centrifuged (1500 rpm) to collect the supernatant. Extraction was repeated for the pellet, supernatant was pooled to make up to 10 ml. Extract aliquots (0.5 ml) were mixed with distilled water (0.5 ml) followed by mixing with Na₂CO₃ (2% in 0.1 N NaOH) (5 ml) and incubated at room temperature (10 min). The Folin–Ciocalteu’s reagent (1:2 v/v dilution with distilled water) (0.5 ml) was added and read the absorbance (725 nm) (UV-VIS Spectrophotometer-118; Systronics, Ahmedabad, Gujarat, India) using tannic acid as standard to express total phenolics in mg of tannic acid equivalents per gram flour (mg TAEs g⁻¹).

ICG (0.3 mg/kg of body weight) was administered intravenously via a peripheral vein. Blood samples were collected at 0, 5-, 10-, 15-, and 20-min post-injection from the contralateral vein. A standard dilution curve was obtained by using varying ICG concentrations. The same was used to estimate the unknown blood ICG levels to measure the dye retention in the liver. The ICG concentrations in standard solution and blood were recorded at 805 nm using a spectrophotometer (Systronics, UV/VIS spectrophotometer118, Gujarat, India). Retention value and plasma disappearance rate were calculated for all the patients. The standard reference ICG retention value was taken as less than 10.0% at 15-min and plasma disappearance rate of more than 18.0 mL/min.