Dtt mix

The medium-sized (10 to 17 µm) C1 chip (Fluidigm) was primed with C1 Harvest Reagent, Preloading Reagent, Blocking Reagent and C1 DNA Seq Cell Wash Buffer (Fluidigm) for 10 min before it was loaded with the dissociated single cells. The DTT Mix was prepared by the addition of DTT, Sample and Reaction Buffers (GE Healthcare). The Lysis Mix contained C1 DNA Seq Lysis Buffer and DTT (Fluidigm), while the Reaction-Enzyme Mix consisted of C1 DNA Seq Reaction Mix (Fluidigm), DTT Mix and Enzyme Mix (GE Healthcare). The Lysis Mix, Reaction-Enzyme Mix and C1 DNA Seq Stop Buffer were loaded on the C1 chip followed by the on-chip whole genome amplification experiment. The amplified DNA was harvested from the C1 chip and transferred into 96-well PCR plate. The DNA was quantified using PicoGreen dsDNA quantification assay (Thermo Fisher) on the Infinite 200Pro plate reader (Tecan).