Axiom genome wide array plate chip system

Whole-genome genotyping was performed on the Biobank participants using the Axiom Genome-Wide Array Plate chip system (Affymetrix Inc., Santa Clara, CA, United States). This study examined SLC2A9 rs3733591 of the urate transporter gene. During quality control, we ensured that the variant rs3733591 had a call rate of >95 percent, a p-value of >0.00001 for the Hardy-Weinberg equilibrium test, and a minor allele frequency of >0.01.

The Axiom Genome-Wide Array Plate chip system (Affymetrix Inc., Santa Clara, CA, USA) TWB (V2.0) chip was used for whole-genome genotyping. We used MTHFR rs1801133 (with genotypes CC, CT, and TT and minor allele T) in the current study because it is a well-established candidate for hypertension in several populations (10 (link), 19 (link), 47 (link)–52 (link)). The Hardy-Weinberg Equilibrium for MTHFR rs1801133 in the control group was 0.5999. The Illumina Infinium Methylation EPIC Bead Chip (Illumina Inc. San Diego, CA, USA) was used to determine whole blood-DNA methylation. We used MTHFR CpG sites at TSS1500 and TSS200 as promoter methylation data in our study (59 (link), 60 (link)). Analysis of methylation data, including normalization, correction for batch effect, and cell-type heterogeneity was done using the ChAMP package.