R v 3.6.0 software

All statistical analyses were performed using R (v.3.6.0) software and SPSS v.24.0 software (IBM Corp.). Comparison of the expression levels of TXNIP between CCRCC and normal groups was performed using an unpaired Student's t-test, and paracancerous groups with a paired t-test. Based on the median value for TXNIP expression (274.17), patients with CCRCC were divided into high- and low-risk groups. Analysis of variance followed by a Least Significance Difference post hoc test and logistic regression were used to analyze the TXNIP expression and pathological parameters of CCRCC. Kaplan-Meier survival analysis was used with the log-rank test to compare the overall survival between the high- and low-TXNIP expression groups. The univariate and multivariate Cox proportional hazards model was used to evaluate the prognostic value of TXNIP expression. P<0.05 was considered to indicate a statistically significant difference.

The relationship between patient clinicopathological parameters and miRNA expression level was assessed by using t-test and Chi-square test, as appropriate. The correlation between three-miRNA signature and patients’ survival was assessed by Kaplan-Meier method. The prognostic value of three miRNAs were analyzed by Cox analysis. All statistical analyses were performed using R (v.3.6.0) software and SPSS v.24.0 software (IBM Corp.).