Mirin

Manufactured by Bio-Techne

Sourced in United Kingdom

Mirin is a laboratory equipment product offered by Bio-Techne. It is a compact, benchtop instrument designed for sample preparation and processing tasks in research and clinical laboratory settings. The core function of Mirin is to provide precise control and automation of various sample handling and preparation steps, such as mixing, aliquoting, and reagent addition.

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Lab products found in correlation

Ku60019, by Bio-Techne (1 mentions) Bx795, by Selleck Chemicals (1 mentions) Hydroxyurea, by Merck Group (1 mentions) Roscovitine, by Bio-Techne (1 mentions)

2 protocols using mirin

Inhibiting Mre11 and ATM for DNA Damage Response

Cited 1 time

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Mirin and KU-60019 were both purchased from Tocris, Bristol, UK. Mirin is a small-molecule inhibitor that blocks the 3ʹ and 5ʹ exonuclease activity associated with Mre11 and prevents ATM activation in response to double-strand breaks (Dupre et al., 2008 (link); Stivers, 2008 (link); Garner et al., 2009 (link)). KU-60019 is a potent ATM kinase inhibitor and has been used to inhibit migration and invasion of human glioma cells in vitro (Golding et al., 2009 (link)).

Tuxworth R.I., Taylor M.J., Martin Anduaga A., Hussien-Ali A., Chatzimatthaiou S., Longland J., Thompson A.M., Almutiri S., Alifragis P., Kyriacou C.P., Kysela B, & Ahmed Z. (2019). Attenuating the DNA damage response to double-strand breaks restores function in models of CNS neurodegeneration. Brain Communications, 1(1), fcz005.

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Drug Treatments and Serum Starvation

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For drug treatment we used: Hydroxyurea (Sigma), Aphidicolin (Calbiochem) at the indicated doses; Roscovitine (Tocris), 50 μM for 5 hours; Mirin (Tocris), 50 μM for 5 hours; BX795 (Selleckchem), 1 μM for 24 hours. For serum starvation experiments, after cell attachment the medium was replaced with low-serum media (0.25% FBS) for serum-starved cells and with fresh media (10% FBS) for asynchronous cells. After 24 h, medium was replaced again (0.25% FBS for serum starved cells; 10% FBS for control cells) until cells were harvested.

Segura-Bayona S., Villamor-Payà M., Attolini C.S., Koenig L.M., Sanchiz-Calvo M., Boulton S.J, & Stracker T.H. (2020). Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of Telomeres. Cell Reports, 32(5), 107983.

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