Frozen tissues were shipped to MOgene, Inc. (St. Louis, MO) on dry ice. High quality RNA was isolated (RNA Integrity Number of 7.5-10 and 28S/18S ratio of 1.6-2.1) and confirmed with an Agilent Bioanalyzer at MOgene, Inc. Five hundred ng of total RNA was amplified using Agilent QuickAmp Labeling Kit (no dye) and purified using Zymo Research RNA Clean and Concentrator spin columns. Three μg of amplified RNA was Cy-labeled using the Kreatech ULS Labeling Kit, fragmented according to Agilent specifications and hybridized to the Agilent sheep gene expression microarray (Agilent Technologies, Santa Clara CA) for 17 hours at 65°C and 10 rpm. Slides were scanned on an Agilent C scanner (20 bit) at 5 μm resolution. Sixteen microarray hybridizations were performed, each with samples from an ANG II, losartan infused fetus (n = 4 for each), or a paired twin control (n = 8), using a mixed design on 2 slides with 8 microarrays per slide. Microarray data were submitted to the NCBI Gene Expression Omnibus (GEO), and can be accessed at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE45463 (project accession no. GSE45463).
Agilent c scanner 20 bit
Manufactured by Agilent Technologies
The Agilent C scanner (20 bit) is a high-resolution imaging device used for various applications. It captures digital images with a bit depth of 20 bits, providing detailed and accurate visual representations. The core function of this scanner is to digitize and convert physical objects or images into a digital format for further analysis or processing.
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Lab products found in correlation
2 protocols using agilent c scanner 20 bit
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Sheep Gene Expression Microarray Analysis
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Sheep Gene Expression Microarray Analysis
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Frozen tissues were shipped to MOgene, Inc. (St. Louis, MO) on dry ice. High quality RNA was isolated (RNA Integrity Number of 7.5-10 and 28S/18S ratio of 1.6-2.1) and confirmed with an Agilent Bioanalyzer at MOgene, Inc. Five hundred ng of total RNA was amplified using Agilent QuickAmp Labeling Kit (no dye) and purified using Zymo Research RNA Clean and Concentrator spin columns. Three μg of amplified RNA was Cy-labeled using the Kreatech ULS Labeling Kit, fragmented according to Agilent specifications and hybridized to the Agilent sheep gene expression microarray (Agilent Technologies, Santa Clara CA) for 17 hours at 65°C and 10 rpm. Slides were scanned on an Agilent C scanner (20 bit) at 5 μm resolution. Sixteen microarray hybridizations were performed, each with samples from an ANG II, losartan infused fetus (n = 4 for each), or a paired twin control (n = 8), using a mixed design on 2 slides with 8 microarrays per slide. Microarray data were submitted to the NCBI Gene Expression Omnibus (GEO), and can be accessed at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE45463 (project accession no. GSE45463).
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