Nod bdc2.5 tcr

NOD mice were purchased from Taconic. NOD.CD45.2 (catalog 014149), NOD.Foxp3^EGFP (catalog 025097) (62 (link)), NOD.BDC2.5 TCR (catalog 004460), and NOD.Rag1^–/– (catalog 003729) mice were purchased from The Jackson Laboratory. NOD.CD90.1 mice were generated by backcrossing BALB/cBy-Thy1a congenic onto NOD/ShiLtJ (catalog 001976) mice for 19 generations and maintained at the University of Minnesota. NOD.8F10 mice were a gift from Emil Unanue (Washington University, St. Louis, Missouri, USA) (28 (link)). NOD.CD45.2^Het.Foxp3^EGFP mice were generated by crossing NOD.CD45.2 mice with NOD.Foxp3^EGFP mice, and F1 mice were used as Treg donors for experiments. NOD.CD90.1.BDC2.5 TCR mice were generated by crossing NOD.CD90.1 mice with NOD.BDC2.5 TCR mice to CD90.1 homozygosity. All mice were housed in specific pathogen–free conditions.

NOD/Sytwu (K^d, D^b, I-A^g7, I-E^null), NOD-Rag1^−/−, and NOD-BDC2.5 TCR transgenic mice were procured from The Jackson Laboratory (Bar Harbor, ME, USA). NOD-SCID mice were purchased from National Laboratory Animal Center (Taipei, Taiwan). All the mice were subsequently housed in specific pathogen-free facility provided by the animal center of National Defense Medical Center (Taipei, Taiwan). Experimental protocols requiring the use of mice were approved by the Institutional Animal Care and Use Committee of National Defense Medical Center.