At the conclusion of the TGFβ time course, cells were counted and resuspended in 500 µL 1% FBS (Cytiva, Marlborough, MA, USA) in PBS with anti-E-cadherin BB700 (BD Biosciences, Franklin Lakes, NJ, USA; #745965, 1:100), anti-CD44 BV421 (BioLegend, San Diego, CA, USA; #562890, 1:100), and anti-CD24 (BioLegend, San Diego, CA, USA; #311104, 1:100), and incubated on ice for 90 min. Following incubation, cells were pelleted and washed twice with 1% FBS in PBS and subjected to flow cytometry using a FACSMelodyTM (BD Biosciences, Franklin Lakes, NJ, USA).
Anti e cadherin bb700
Manufactured by BD
Sourced in United States
The Anti-E-cadherin BB700 is a fluorochrome-conjugated monoclonal antibody that specifically binds to the E-cadherin protein. E-cadherin is a cell-cell adhesion molecule that plays a crucial role in the maintenance of cell-cell junctions and epithelial tissue architecture. The Anti-E-cadherin BB700 can be used for the detection and analysis of E-cadherin expression in various cell and tissue samples using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
2 protocols using anti e cadherin bb700
1
E-cadherin, CD44, and CD24 Analysis
2
Flow Cytometry Analysis of EMT Markers
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At the conclusion of the TGFβ time course, cells were counted and resuspended in 500 µL 1% FBS (Cytiva) in PBS with anti-E-cadherin BB700 (BD Biosciences #745965, 1:100), anti-CD44 BV421 (BioLegend #562890, 1:100), and anti-CD24 (BioLegend #311104, 1:100) and incubated on ice for 90 minutes. Following incubation, cells were pelleted and washed twice with 1% FBS in PBS and subjected to flow cytometry using a FACSMelody TM (BD Biosciences).
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