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3 protocols using sodium deoxycholate

1

Development and Evaluation of Cholesterol Nanodispersion

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Unicure Pvt. Limited (Noida, India) provided CIP. The cholesterol (CHO), Span 60, and sodium deoxycholate (SDC) were procured from SD Fine Chemicals (Mumbai, India). Acetonitrile, chloroform, methanol, and dialysis bag (MWCO 12kDa) were procured from Sigma Aldrich (Bengaluru, India). Carbopol 934P, Hydroxy propyl methyl cellulose K100 M (HPMC-K100M), Sodium Hydroxide (NaOH), Calcium chloride (CaCl2), Sodium Bicarbonate (NaHCO3), and Sodium Chloride (NaCl) were procured from SD fine Chem Limited (Mumbai, Maharashtra, India). Fluid thioglycolate and soya bean casein digest media were obtained from HiMedia (Mumbai, India). The other chemicals utilized in this research are analytical grade.
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2

Nanoformulation Preparation and Characterization

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BBR,
CUR, acetonitrile,
methanol, and ethanol were obtained from Sigma-Aldrich (Mumbai, India).
Sodium deoxycholate (SDC) was purchased from SD Fine Chemicals (Mumbai,
India). Span 60 was received from Sigma-Aldrich (Mumbai, India). A
dialysis cellulose membrane with a 5000 Da molecular weight cut off
was received from Sigma-Aldrich Chemie GmbH (Munich, Germany). Membrane
filters were purchased from EMD Millipore Company (Cork, Ireland).
Disodium hydrogen phosphate and potassium dihydrogen phosphate were
purchased from SISCO Research Laboratories (Mumbai, India). Xylene
was procured from El-Nasr Pharmaceutical Co. (Cairo, Egypt). Transmission
electron microscopy (TEM) grids coated with carbon were procured from
EMS (CF300-Cu, Hatfield, PA). The acetylcholinesterase activity assay
kit (Cat. no.: MAK119) was procured from Sigma-Aldrich (St. Louis,
MO). DAPI (4′,6-diamidino-2-phenylindole) was obtained from
Sigma-Aldrich (St. Louis, MO). Hydrogen peroxide and potassium dichromate
(protein estimation kit, Bradford GeNei) were purchased from Merck
(Darmstadt, Germany). Acetic acid, ammonium chloride, sodium dihydrogenphosphate,
potassium chloride, and Disodium hydrogen phosphate were purchased
from Sisco Laboratory Limited (Mumbai, India).
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3

Quercetin-Chitosan Antioxidant Formulation

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Quercetin, Chitosan (deacetylation degree ≥ 95%, viscosity 100–200 mpa.s), chloroform, methanol, and acetonitrile were procured from Sigma Aldrich (Bengaluru, India). Span 60 and sodium deoxycholate (SDC) were acquired from SD fine Chemicals (Mumbai, India). 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ABTH were obtained from Sigma Chemical Co. (St. Louis, MO, USA). The rest of the chemicals utilized in this experiment are of analytical grade.
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